Overview

  • Product name
    Anti-TIMP2 antibody [3A4]
    See all TIMP2 primary antibodies
  • Description
    Mouse monoclonal [3A4] to TIMP2
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Guinea pig, Cow, Human
  • Immunogen

    Synthetic peptide corresponding to Human TIMP2 (N terminal).

  • Positive control
    • IHC-P: Normal human placenta tissue.
  • General notes

    This antibody clone is manufactured by Abcam. If you require it in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

Properties

Applications

Our Abpromise guarantee covers the use of ab1828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr 1/100. PubMed: 24343133

Target

Images

  • IHC image of TIMP2 staining in a section of formalin-fixed paraffin-embedded normal human placenta* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab1828, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References

This product has been referenced in:
  • Huang D  et al. TPX2 silencing mediated by joint action of microvesicles and ultrasonic radiation inhibits the migration and invasion of SKOV3 cells. Mol Med Rep 17:7627-7635 (2018). Read more (PubMed: 29620263) »
  • Mota R  et al. Increasing Cardiomyocyte Atrogin-1 Reduces Aging-Associated Fibrosis and Regulates Remodeling in Vivo. Am J Pathol 188:1676-1692 (2018). Read more (PubMed: 29758183) »
See all 30 Publications for this product

Customer reviews and Q&As

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1-7 of 7 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Sample
Human Tissue sections (Ascending aorta)
Specification
Ascending aorta
Permeabilization
No
Fixative
Formaldehyde

Mr. Mc Shen

Verified customer

Submitted Feb 05 2015

Application
Western blot
Sample
Rat Tissue lysate - whole (ovaries)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
100 µg
Specification
ovaries
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10%

Abcam user community

Verified customer

Submitted Sep 30 2013

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Astrocytes)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
50 µg
Specification
Astrocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Dec 03 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0,1% Triton X
Specification
HeLa
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Methanol

Abcam user community

Verified customer

Submitted Nov 29 2012

Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal Muscle)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
50 µg
Specification
Skeletal Muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Dr. Steve John

Verified customer

Submitted Aug 07 2012

Application
Western blot
Sample
Rat Cell lysate - whole cell (mesenchymal stem cells)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
15 µg
Specification
mesenchymal stem cells
Blocking step
Milk as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Aug 20 2009

Application
Western blot
Sample
Rat Cell lysate - other (L6 Cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
L6 Cell
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 05 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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