The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/25 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100. PubMed: 24343133
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
Belongs to the protease inhibitor I35 (TIMP) family. Contains 1 NTR domain.
The activity of TIMP2 is dependent on the presence of disulfide bonds.
Huang D et al. TPX2 silencing mediated by joint action of microvesicles and ultrasonic radiation inhibits the migration and invasion of SKOV3 cells. Mol Med Rep17:7627-7635 (2018).
Read more (PubMed: 29620263) »
Wang L et al. Correlation between Tissue Characterization and Dynamic Expression of Matrix Metalloproteinase-2 and Its Tissue Inhibitor in Conjunctival Filtering Bleb of Rats. Biomed Res Int2017:1054129 (2017).
Read more (PubMed: 29075637) »