Overview

  • Product name
    Anti-TIMP2 antibody [3A4]
    See all TIMP2 primary antibodies
  • Description
    Mouse monoclonal [3A4] to TIMP2
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Guinea pig, Cow, Human
  • Immunogen

    BALB/C mice were injected with a synthetic peptide from the N-terminal region of human TIMP 2.

  • Positive control
    • IHC-P: Human placenta tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab1828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr 1/100. PubMed: 24343133

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling TIMP2 with ab1828.

References

This product has been referenced in:
  • Huang D  et al. TPX2 silencing mediated by joint action of microvesicles and ultrasonic radiation inhibits the migration and invasion of SKOV3 cells. Mol Med Rep 17:7627-7635 (2018). Read more (PubMed: 29620263) »
  • Wang L  et al. Correlation between Tissue Characterization and Dynamic Expression of Matrix Metalloproteinase-2 and Its Tissue Inhibitor in Conjunctival Filtering Bleb of Rats. Biomed Res Int 2017:1054129 (2017). Read more (PubMed: 29075637) »

See all 24 Publications for this product

Customer reviews and Q&As

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Sample
Human Tissue sections (Ascending aorta)
Specification
Ascending aorta
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Mc Shen

Verified customer

Submitted Feb 05 2015

Application
Western blot
Sample
Rat Tissue lysate - whole (ovaries)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
100 µg
Specification
ovaries
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

Submitted Sep 30 2013

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Astrocytes)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
50 µg
Specification
Astrocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Dec 03 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0,1% Triton X
Specification
HeLa
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Methanol
Username

Abcam user community

Verified customer

Submitted Nov 29 2012

Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal Muscle)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
50 µg
Specification
Skeletal Muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Dr. Steve John

Verified customer

Submitted Aug 07 2012

Application
Western blot
Sample
Rat Cell lysate - whole cell (mesenchymal stem cells)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
15 µg
Specification
mesenchymal stem cells
Blocking step
Milk as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Aug 20 2009

Application
Western blot
Sample
Rat Cell lysate - other (L6 Cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
L6 Cell
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 05 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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