Overview

  • Product name
  • Description
    Rabbit polyclonal to TIMP2
  • Host species
    Rabbit
  • Specificity
    ab53730 detects endogenous levels of total TIMP2 protein.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from human TIMP2.

  • Positive control
    • A549 cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab53730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 24 kDa).
ELISA 1/40000.

Target

  • Function
    Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
  • Sequence similarities
    Belongs to the protease inhibitor I35 (TIMP) family.
    Contains 1 NTR domain.
  • Post-translational
    modifications
    The activity of TIMP2 is dependent on the presence of disulfide bonds.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CSC 21K antibody
    • CSC-21K antibody
    • CSC21K antibody
    • Metalloproteinase inhibitor 2 antibody
    • Metalloproteinase inhibitor 2 precursor antibody
    • TIMP 2 antibody
    • TIMP metallopeptidase inhibitor 2 antibody
    • TIMP-2 antibody
    • TIMP2 antibody
    • TIMP2_HUMAN antibody
    • Tissue Inhibitor of Metalloproteinase 2 antibody
    • Tissue inhibitor of metalloproteinases 2 antibody
    see all

Images

  • All lanes : Anti-TIMP2 antibody (ab53730) at 1/500 dilution

    Lane 1 : A549 cell extract
    Lane 2 : A549 cell extract with immunising peptide

    Predicted band size: 24 kDa
    Observed band size: 31 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:
  • Hussain H  et al. A protein chimera strategy supports production of a model "difficult-to-express" recombinant target. FEBS Lett 592:2499-2511 (2018). Read more (PubMed: 29933498) »
  • Sharif R  et al. Collagen cross-linking impact on keratoconus extracellular matrix. PLoS One 13:e0200704 (2018). Read more (PubMed: 30020979) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your patience.
The specific immunogen information is considered proprietary, but ab53730 binds to the NTR domain of the protein. I hope this helps, please let me know if you need any additional information or assistance.

Read More

Answer

Thank you for contacting us.
While the immunogen sequences for our antibodies are considered proprietary, we do provide general information about the immunogen range on our datasheets:
ab38972: Synthetic peptide based on the Human sequence. The antibody epitope is located in loop 3 of TIMP2. The antibody does not cross-react with TIMP1, TIMP3, or TIMP4.
ab38973: Synthetic peptide based on Human TIMP2 sequence. The epitope is located in loop #1 of TIMP2. The antibody does not cross-react with TIMP1, TIMP3, or TIMP4.
ab53730: Synthetic peptide derived from human TIMP2.
ab28260: This antibody is made against a synthetic peptide based on loop 2 of human TIMP2, which may be more bioavailable than the amino terminal or carboxy terminal locations. The antibody does not cross-react with TIMP1, TIMP3, or TIMP4.
ab74169: Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human IGFBP7
We are also unable to disclose the sequence for peptide ab41149 because it was used as the immunogen for ab38973.
I hope this helps, please let me know if you need any additional information or assistance.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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