Overview

  • Product name

  • Description

    Rabbit polyclonal to TIMP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human TIMP2 aa 200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab86642)

  • Positive control

    • Recombinant human TIMP2 protein (ab82117) can be used as a positive control in WB. This antibody gave a positive signal in Rat Skeletal Muscle Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab64040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).

Target

  • Function

    Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
  • Sequence similarities

    Belongs to the protease inhibitor I35 (TIMP) family.
    Contains 1 NTR domain.
  • Post-translational
    modifications

    The activity of TIMP2 is dependent on the presence of disulfide bonds.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • CSC 21K antibody
    • CSC-21K antibody
    • CSC21K antibody
    • Metalloproteinase inhibitor 2 antibody
    • Metalloproteinase inhibitor 2 precursor antibody
    • TIMP 2 antibody
    • TIMP metallopeptidase inhibitor 2 antibody
    • TIMP-2 antibody
    • TIMP2 antibody
    • TIMP2_HUMAN antibody
    • Tissue Inhibitor of Metalloproteinase 2 antibody
    • Tissue inhibitor of metalloproteinases 2 antibody
    see all

Images

  • Anti-TIMP2 antibody (ab64040) at 1 µg/ml + Skeletal Muscle (Rat) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 24 kDa
    Observed band size: 24 kDa
    Additional bands at: 180 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:

  • Zhang X  et al. NLRP3 Inflammasome Is Involved in Calcium-Sensing Receptor-Induced Aortic Remodeling in SHRs. Mediators Inflamm 2019:6847087 (2019). Read more (PubMed: 30906225) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Airway epithelial cells)
Specification
Airway epithelial cells
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Sep 07 2012

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