Overview

  • Product name

  • Description

    Rabbit polyclonal to TIMP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TIMP2 aa 50-150 (N terminal). The exact sequence is proprietary.
    Database link: P16035

  • Positive control

    • Paraffin embedded Human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab74216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
WB Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.

Target

  • Function

    Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
  • Sequence similarities

    Belongs to the protease inhibitor I35 (TIMP) family.
    Contains 1 NTR domain.
  • Post-translational
    modifications

    The activity of TIMP2 is dependent on the presence of disulfide bonds.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • CSC 21K antibody
    • CSC-21K antibody
    • CSC21K antibody
    • Metalloproteinase inhibitor 2 antibody
    • Metalloproteinase inhibitor 2 precursor antibody
    • TIMP 2 antibody
    • TIMP metallopeptidase inhibitor 2 antibody
    • TIMP-2 antibody
    • TIMP2 antibody
    • TIMP2_HUMAN antibody
    • Tissue Inhibitor of Metalloproteinase 2 antibody
    • Tissue inhibitor of metalloproteinases 2 antibody
    see all

Images

  • ab74216 at 1/50 dilution staining TIMP2 in human colon carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.

References

This product has been referenced in:

  • Serralheiro P  et al. Variability of MMP/TIMP and TGF-ß1 Receptors throughout the Clinical Progression of Chronic Venous Disease. Int J Mol Sci 19:N/A (2017). Read more (PubMed: 29267209) »
See 1 Publication for this product

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