Anti-TIMP2 antibody [F27P3A4] (ab230511)
Key features and details
- Mouse monoclonal [F27P3A4] to TIMP2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-TIMP2 antibody [F27P3A4]
See all TIMP2 primary antibodies -
Description
Mouse monoclonal [F27P3A4] to TIMP2 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog -
Immunogen
Synthetic peptide corresponding to Human TIMP2 aa 50-150 conjugated to ovalbumin.
Database link: P16035 -
Positive control
- IHC-P: Human breast tissue. WB: Recombinant human TIMP2 protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from TCS? -
Clonality
Monoclonal -
Clone number
F27P3A4 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml.
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IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. |
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. -
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
Post-translational
modificationsThe activity of TIMP2 is dependent on the presence of disulfide bonds. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 374178 Chicken
- Entrez Gene: 282093 Cow
- Entrez Gene: 403633 Dog
- Entrez Gene: 100135629 Guinea pig
- Entrez Gene: 100034134 Horse
- Entrez Gene: 7077 Human
- Entrez Gene: 21858 Mouse
- Entrez Gene: 29543 Rat
see all -
Alternative names
- CSC 21K antibody
- CSC-21K antibody
- CSC21K antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab230511 has been referenced in 2 publications.
- Chen D et al. circRNA DENND1B inhibits tumorigenicity of clear cell renal cell carcinoma via miR-122-5p/TIMP2 axis. Open Med (Wars) 17:2085-2097 (2022). PubMed: 36578555
- Liu D et al. HNRNPA1-mediated exosomal sorting of miR-483-5p out of renal tubular epithelial cells promotes the progression of diabetic nephropathy-induced renal interstitial fibrosis. Cell Death Dis 12:255 (2021). PubMed: 33692334