Overview

  • Product name
  • Description
    Rabbit polyclonal to TIMP3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Rabbit, Human, Baboon
  • Immunogen

    Synthetic peptide from the C-terminus region of the human TIMP-3.

  • Positive control
    • Breast carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab2169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-P
IHC-Fr
  • Application notes
    ICC/IF: Use at an assay dependent dilution (PMID 19324423).
    IHC-P: Use at an assay dependent concentration.
    IHC-Fr: Use at an assay dependent concentration.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.
    • Involvement in disease
      Defects in TIMP3 are the cause of Sorsby fundus dystrophy (SFD) [MIM:136900]. SFD is a rare autosomal dominant macular disorder with an age of onset in the fourth decade. It is characterized by loss of central vision from subretinal neovascularization and atrophy of the ocular tissues. Generally, macular disciform degeneration develops in the patients eye within 6 months to 6 years.
    • Sequence similarities
      Belongs to the protease inhibitor I35 (TIMP) family.
      Contains 1 NTR domain.
    • Cellular localization
      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • HSMRK222 antibody
      • K222 antibody
      • K222TA2 antibody
      • Metalloproteinase inhibitor 3 antibody
      • MIG 5 protein antibody
      • MIG5 protein antibody
      • Protein MIG 5 antibody
      • Protein MIG-5 antibody
      • SFD antibody
      • Sorsby fundus dystrophy pseudoinflammatory antibody
      • TIMP 3 antibody
      • TIMP metallopeptidase inhibitor 3 antibody
      • TIMP-3 antibody
      • TIMP3 antibody
      • TIMP3_HUMAN antibody
      • Tissue Inhibitor of Metalloproteinase 3 antibody
      • Tissue inhibitor of metalloproteinases 3 antibody
      • Tissue inhibitor of metalloproteinases3 antibody
      see all

    References

    This product has been referenced in:
    • Hurst LA  et al. Expression of ADAM-17, TIMP-3 and fractalkine in the human adult brain endothelial cell line, hCMEC/D3, following pro-inflammatory cytokine treatment. J Neuroimmunol 210:108-12 (2009). ICC/IF ; Human . Read more (PubMed: 19324423) »
    • Sun X  et al. Molecular characterization of tumour heterogeneity and malignant mesothelioma cell differentiation by gene profiling. J Pathol 207:91-101 (2005). Read more (PubMed: 16007577) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Answer

    Thank you for your enquiry. The immunogen sequence is proprietary and we have not determined the homology to mouse. We would suggest comparing the C-terminus regions of human and mouse TIMP3. Please contact us again if you have any additional questions.

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    Answer

    Thank you for your enquiry. For ab11814 I regret to inform you that the antibody has not yet been tested for application in IHC. The antibody has only so far been characterized for application in Western blotting and I have updated the online datasheet to reflect this information. For ab2169, the immunogen used was a synthetic peptide from the C-terminus region of the human TIMP-3. The exact sequence is proprietary. The concentration of the current batch is 100 ug/ml. If you have any additional questions, please contact us again.

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    Question
    Answer

    I'm sorry to hear you are having a problem with aab2169 in WB. We have not tested this antibody in western blotting and it is possible that the antibody does not work in this application, or that it requires the protein to be in its native form. I would therefore like to suggest to run your samples in non-reducing (and maybe also non denaturing) conditions to test this possibility. You mention HEK cells as positive control and placenta tissue; 293 cell lysate and the choriocarcinoma cell lines JAR, JEG-3, B6 should contain high levels of the protein (according to Feng H et al, Gynecol Oncol. 2004 Aug;94(2):375-82) however I did not find a reference for normal placental tissue containing high levels of TIMP3. We have used human breast carcinoma tisseu as positive control for IHC. The lysis buffer used in the paper by Feng et al is SDS lysis buffer [50 mM Tris–HCl (pH 6.8), 2% SDS, 10% glycerol], containing proteinase inhibitors (1 µg/ml aprotinin, 1 µg/ml leupeptin, 1 mM phenylmethylsulfonyl fluoride), is this the same as the one you used? If this is not similar the problem may also be due to an inadequate extraction of the protein. Finally, another possible problem may be the blocking buffer you are currently using. I would like to suggest blocking the membrane for 1hr in 5%BSA in TBST, then rinsing the membrane in TBST for 2 seconds and incubating ab2169 in TBST only. Between steps I would recommend washing in TBST only too and to incubate the secondary in TBST too. Please let me know if this helps and do not hesitate to contact us for further advice,

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue lysate - whole (Adult human skin)
    Specification
    Adult human skin
    Fixative
    Methanol
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: .02

    Abcam user community

    Verified customer

    Submitted Aug 26 2005

    Answer

    Thank you for your enquiry and for your patience. Regarding ab2169, it has not been tested for cross-reactivity with TIPM 1,2 or 3. However, we would be more than happy to provide you the peptide sequence for you to see if there is any homology with TIMP 1,2 or 3 if you are interested. For ab2169, the antibody has been reported to work in IHC but has not specifically been tested yet in paraffin embedded sections. The antibody's originator has suggested first trying frozen sections fixed with formalin before trying paraffin embedded tissue. If you have any more questions, please contact us again.

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    Answer

    We do not know its crossreactivity with mouse. However, it does not cross react with rat, cow, pig and bird. On the other hand, it does crossreact with rabbit and baboon.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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