Product nameAnti-TIMP3 antibody
See all TIMP3 primary antibodies
DescriptionRabbit polyclonal to TIMP3
Specificityab39184 binds to TIMP3. It recognizes the glycosylated and unglycosylated forms of TIMP3, and works against native or reduced TIMP3. Ab39184 does not cross react with the other TIMP family members (TIMP1, TIMP2, TIMP4).
Tested applicationsSuitable for: ICC/IF, WB, IHC-P, IHC-Frmore details
Species reactivityReacts with: Mouse, Rat, Human
- Human and mouse TIMP3 recombinant protein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab39184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
|WB||1/1000 - 1/5000. Detects a band of approximately 24, 30 kDa (predicted molecular weight: 24 kDa). Dilution optimised using Chromogenic detection.|
|IHC-P||Use at an assay dependent concentration. PubMed: 20448060
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionComplexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.
Involvement in diseaseDefects in TIMP3 are the cause of Sorsby fundus dystrophy (SFD) [MIM:136900]. SFD is a rare autosomal dominant macular disorder with an age of onset in the fourth decade. It is characterized by loss of central vision from subretinal neovascularization and atrophy of the ocular tissues. Generally, macular disciform degeneration develops in the patients eye within 6 months to 6 years.
Sequence similaritiesBelongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- HSMRK222 antibody
- K222 antibody
- K222TA2 antibody
All lanes : Anti-TIMP3 antibody (ab39184)
Lane 1 : Human TIMP3
Lane 2 : Mouse TIMP3
Lane 3 : crude control (preparation of extracellular matrix from BHK cells producing recombinant human TIMP-3)
Predicted band size: 24 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
ICC/IF image of ab39184 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39184, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab39184 staining TIMP3 in mouse fallopian tube tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with PBST (PBS / 0.1% v/v Triton X-100) and blocked with 3% donkey serum / 1% BSA in PBST for 1 hour at 25°C. Samples were incubated with primary antibody (1/200 in blocking buffer) for 48 hours at 25°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
Positive TIMP3 staining is seen in the extracellular space. The image shows DAPI counterstain, red field (autofluorescence), green field antibody staining and overlay.
This product has been referenced in:
- Wymeersch FJ et al. Transcriptionally dynamic progenitor populations organised around a stable niche drive axial patterning. Development 146:N/A (2019). Read more (PubMed: 30559277) »
- Cheng Y et al. TIMP-3 suppression induces choroidal neovascularization by moderating the polarization of macrophages in age-related macular degeneration. Mol Immunol 106:119-126 (2019). Read more (PubMed: 30594674) »