Anti-TIMP4 antibody (ab38987)
Key features and details
- Rabbit polyclonal to TIMP4
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TIMP4 antibody
See all TIMP4 primary antibodies -
Description
Rabbit polyclonal to TIMP4 -
Host species
Rabbit -
Specificity
Ab38987 recognises the carboxiterminal region of TIMP4. It does not react with other TIMPs. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide based on the carboxyterminal region of human TIMP4.
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Positive control
- Human TIMP4, cell media from human colorectal adenosarcoma and rat heart.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituent: 2.9% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab38987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 26 kDa).
Recommended starting dilution of 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 when using chemiluminescent substrates. Predicted molecular weight: 26 kDa. When used against the reduced protein, ab38987 identifies bands at 23 and 29 kDa, representing the unglycosylated and glycosylated forms of TIMP4 respectively. Treatment of cells with the phorbol ester TPA stimulates production of TIMP4 in some cell types but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting. Dilution optimised using Chromogenic detection. |
Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 26 kDa). Recommended starting dilution of 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 when using chemiluminescent substrates. Predicted molecular weight: 26 kDa. When used against the reduced protein, ab38987 identifies bands at 23 and 29 kDa, representing the unglycosylated and glycosylated forms of TIMP4 respectively. Treatment of cells with the phorbol ester TPA stimulates production of TIMP4 in some cell types but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting. Dilution optimised using Chromogenic detection. |
Target
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Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9. -
Tissue specificity
Abundant in heart and present at low levels in many other tissues. -
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 7079 Human
- Entrez Gene: 680130 Rat
- Omim: 601915 Human
- SwissProt: Q99727 Human
- SwissProt: P81556 Rat
- Unigene: 591665 Human
- Unigene: 155651 Rat
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Alternative names
- Metalloproteinase inhibitor 4 antibody
- TIMP 4 antibody
- TIMP metallopeptidase inhibitor 4 antibody
see all
Images
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All lanes : Anti-TIMP4 antibody (ab38987) at 1/1000 dilution
Lane 1 : human TIMP4
Lane 2 : cell media from human colorectal adenosarcoma
Lane 3 : rat heart
Predicted band size: 26 kDa
Observed band size: 25,45,66,97 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa, 23 kDa (possible glycosylated form). We are unsure as to the identity of these extra bands.
Datasheets and documents
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Datasheet download
References (2)
ab38987 has been referenced in 2 publications.
- Corporan D et al. Temporal changes in myocardial collagen, matrix metalloproteinases, and their tissue inhibitors in the left ventricular myocardium in experimental chronic mitral regurgitation in rodents. Am J Physiol Heart Circ Physiol 315:H1269-H1278 (2018). PubMed: 30141979
- Horn MA et al. Age-related divergent remodeling of the cardiac extracellular matrix in heart failure: collagen accumulation in the young and loss in the aged. J Mol Cell Cardiol 53:82-90 (2012). WB ; Sheep . PubMed: 22516365