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Rabbit polyclonal to TIMP4
ab58425 detects endogenous levels of total TIMP4 protein.
Mouse, Rat, Human
Synthetic peptide derived from C terminal residues of human TIMP4
- IHC-P: Human lung carcinoma tissue.
WB: NIH3T3 cell extracts.
Store at -20°C. Stable for 12 months at -20°C
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2
Concentration information loading...
Immunogen affinity purified
ab58425 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use at an assay dependent concentration. PubMed: 23525608
||Use at an assay dependent concentration.
||1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9.
Abundant in heart and present at low levels in many other tissues.
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
Information by UniProt
- Metalloproteinase inhibitor 4 antibody
- TIMP 4 antibody
- TIMP metallopeptidase inhibitor 4 antibody
Western blot - Anti-TIMP4 antibody (ab58425)
All lanes : Anti-TIMP4 antibody (ab58425) at 1/500 dilution
Lane 1 : NIH3T3 cell extract
Lane 2 : NIH3T3 cell extract with immunising peptide
Predicted band size: 26 kDa
Observed band size: 26 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP4 antibody (ab58425)
ab58425, at 1/50 dilution, staining TIMP4 in paraffin embedded human lung carcinoma tissue by Immunohistochemistry, in the absence (left image) or presence (right image) of the immunising peptide.
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