Overview

  • Product name

    Anti-Tin2 antibody [EPR15319]
    See all Tin2 primary antibodies
  • Description

    Rabbit monoclonal [EPR15319] to Tin2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Tin2 aa 200-350. The exact sequence is proprietary.
    Database link: Q9BSI4

  • Positive control

    • MCF7, WI38 and HUVEC cell lysate. Mouse brain, heart, kidney and spleen lysate. C6, RAW264.7, PC12 and NIH3T3 cell lysate. Human, mouse and rat thymus lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab197894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 50 kDa).

Target

  • Function

    Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Plays a role in shelterin complex assembly.
  • Tissue specificity

    Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Involvement in disease

    Defects in TINF2 are a cause of dyskeratosis congenita autosomal dominant (ADDKC) [MIM:127550]; also known as dyskeratosis congenita Scoggins type. ADDKC is a rare, progressive bone marrow failure syndrome characterized by the triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.
    Defects in TINF2 are a cause of retinopathy exudative with bone marrow failure (ERBMF) [MIM:268130]; also known as Revesz syndrome. ERBMF is characterized by bilateral exudative retinopathy, bone marrow hypoplasia, nail dystrophy, fine hair, cerebellar hypoplasia, and growth retardation.
  • Domain

    The TBM domain mediates interaction with TERF1.
  • Cellular localization

    Nucleus. Chromosome > telomere. Associated with telomeres.
  • Information by UniProt
  • Database links

  • Alternative names

    • AW552114 antibody
    • D14Wsu146e antibody
    • DKCA3 antibody
    • MGC94711 antibody
    • TERF 1 (TRF 1) interacting nuclear factor 2 antibody
    • TERF 1 interacting nuclear factor 2 antibody
    • TERF1 (TRF1) interacting nuclear factor 2 antibody
    • TERF1 interacting nuclear factor 2 antibody
    • TERF1-interacting nuclear factor 2 antibody
    • Tin 2 antibody
    • TIN2 antibody
    • TINF 2 antibody
    • Tinf2 antibody
    • TINF2_HUMAN antibody
    • TRF 1 interacting nuclear factor 2 antibody
    • TRF1 interacting nuclear factor 2 antibody
    • TRF1-interacting nuclear protein 2 antibody
    see all

Images

  • All lanes : Anti-Tin2 antibody [EPR15319] (ab197894) at 1/10000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 2 : WI38 (Human fetal lung fibroblast cells) whole cell lysate
    Lane 3 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 39 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    5% NFDM/TBST: Blocking and diluting buffer.

  • All lanes : Anti-Tin2 antibody [EPR15319] (ab197894) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate
    Lane 5 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 6 : RAW264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 7 : PC12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 8 : NIH3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 38 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    5% NFDM/TBST: Blocking and diluting buffer.

  • All lanes : Anti-Tin2 antibody [EPR15319] (ab197894) at 1/2000 dilution

    Lane 1 : Human thymus lysate
    Lane 2 : Mouse thymus lysate
    Lane 3 : Rat thymus lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 38 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    5% NFDM/TBST: Blocking and diluting buffer.

References

This product has been referenced in:

  • Yang H  et al. Concomitant underexpression of TGFBR2 and overexpression of hTERT are associated with poor prognosis in cervical cancer. Sci Rep 7:41670 (2017). WB ; Human . Read more (PubMed: 28195144) »
  • Tan R  et al. Nek7 Protects Telomeres from Oxidative DNA Damage by Phosphorylation and Stabilization of TRF1. Mol Cell 65:818-831.e5 (2017). Read more (PubMed: 28216227) »
See all 3 Publications for this product

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