Overview

  • Product name

  • Description

    Rabbit polyclonal to TIP150
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment within Human TIP150 (internal sequence). The exact sequence is proprietary.
    Database link: Q5JR59

  • Positive control

    • WB: U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts. IHC-P: Rat hind brain tissue.
  • General notes

    Previously labelled as MTUS2. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 150 kDa.

Target

  • Function

    Binds microtubules. Together with MAPRE1 may target the microtubule depolymerase KIF2C to the plus-end of microtubules. May regulate the dynamics of microtubules at their growing distal tip.
  • Tissue specificity

    Detected in embryonic stem cells differentiating to cardiomyocytes.
  • Sequence similarities

    In the C-terminal section; belongs to the MTUS1 family.
  • Cellular localization

    Cytoplasm > cytoskeleton. Associated with the microtubule network at the growing distal tip (the plus-end) of microtubules.
  • Information by UniProt
  • Database links

  • Alternative names

    • +TIP of 150 kDa antibody
    • 5730592G18Rik antibody
    • A730013O20Rik antibody
    • AU067634 antibody
    • C130038G02Rik antibody
    • Cardiac zipper protein antibody
    • CAZIP antibody
    • Gm763 antibody
    • KIAA0774 antibody
    • Microtubule plus-end tracking protein TIP150 antibody
    • Microtubule-associated tumor suppressor candidate 2 antibody
    • mKIAA0774 antibody
    • Mtus2 antibody
    • MTUS2_HUMAN antibody
    • OTTHUMP00000018187 antibody
    • OTTHUMP00000018188 antibody
    • RP11-274A8.2 antibody
    • TIP150 antibody
    • Tracking protein of 150 kDa antibody
    see all

Images

  • All lanes : Anti-TIP150 antibody (ab229121) at 1/1000 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
    Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
    Lane 3 : IMR32 whole cell extract
    Lane 4 : SK-N-AS whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 150 kDa



    5% SDS-PAGE gel.

  • Paraffin-embedded rat hind brain tissue stained for TIP150 with ab229121 at 1/500 dilution in immunohistochemical analysis.

References

ab229121 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229121.
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