Key features and details
- Rabbit polyclonal to TIP150
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-TIP150 antibody
See all TIP150 primary antibodies
DescriptionRabbit polyclonal to TIP150
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Cow
Recombinant fragment within Human TIP150 (internal sequence). The exact sequence is proprietary.
Database link: Q5JR59
- WB: U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts. IHC-P: Rat hind brain tissue.
Previously labelled as MTUS2.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229121 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 150 kDa.|
FunctionBinds microtubules. Together with MAPRE1 may target the microtubule depolymerase KIF2C to the plus-end of microtubules. May regulate the dynamics of microtubules at their growing distal tip.
Tissue specificityDetected in embryonic stem cells differentiating to cardiomyocytes.
Sequence similaritiesIn the C-terminal section; belongs to the MTUS1 family.
Cellular localizationCytoplasm > cytoskeleton. Associated with the microtubule network at the growing distal tip (the plus-end) of microtubules.
- Information by UniProt
- +TIP of 150 kDa antibody
- 5730592G18Rik antibody
- A730013O20Rik antibody
All lanes : Anti-TIP150 antibody (ab229121) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 3 : IMR32 whole cell extract
Lane 4 : SK-N-AS whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 150 kDa
5% SDS-PAGE gel.
Paraffin-embedded rat hind brain tissue stained for TIP150 with ab229121 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229121 has not yet been referenced specifically in any publications.