Recombinant Anti-TIP30 antibody [EPR13179] - BSA and Azide free (ab250031)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13179] to TIP30 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TIP30 antibody [EPR13179] - BSA and Azide free
See all TIP30 primary antibodies -
Description
Rabbit monoclonal [EPR13179] to TIP30 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250031 is the carrier-free version of ab177961.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13179 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 27 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Notes |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 27 kDa. |
ICC/IF
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
Target
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Function
Oxidoreductase required for tumor suppression. NAPDH-bound form inhibits nuclear import by competing with nuclear import substrates for binding to a subset of nuclear transport receptors. May act as a redox sensor linked to transcription through regulation of nuclear import. Isoform 1 is a metastasis suppressor with proapoptotic as well as antiangiogenic properties. Isoform 2 has an antiapoptotic effect. -
Tissue specificity
Ubiquitous. Highest level in liver. High levels in lung, skeletal muscle, pancreas and placenta. Moderate levels in heart and kidney. Low levels in brain. Not expressed or low levels in variant small cell lung carcinomas, 33% of hepatocellular carcinomas and neuroblastomas. -
Domain
Unique C-terminus confers high proteasome-dependent instability to isoform 2. -
Cellular localization
Cytoplasm. Nucleus envelope. - Information by UniProt
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Database links
- Entrez Gene: 10553 Human
- Entrez Gene: 53415 Mouse
- Entrez Gene: 292935 Rat
- Omim: 605628 Human
- SwissProt: Q9BUP3 Human
- SwissProt: Q9Z2G9 Mouse
- Unigene: 90753 Human
- Unigene: 20801 Mouse
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Alternative names
- 30 kDa HIV 1 TAT interacting protein antibody
- 30 kDa HIV-1 TAT-interacting protein antibody
- 30 kDa HIV1 TAT interacting protein antibody
see all
Images
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All lanes : Anti-TIP30 antibody [EPR13179] (ab177961) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Fetal liver lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 27 kDaThis data was developed using ab177961, the same antibody clone in a different buffer formulation.
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This data was developed using ab177961, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Human placenta tissue labeling TIP30 using ab177961 at 1/50 dilution. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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This data was developed using ab177961, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Human pancreas tissue labeling TIP30 using ab177961 at 1/50 dilution. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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This data was developed using ab177961, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from (1) HeLa cell lysate or (2) 1XPBS (negative control) using ab177961 at 1/10 dilution to immunoprecipitate TIP30, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250031 has not yet been referenced specifically in any publications.