Product nameAnti-TIP30 antibody [EPR13185]
See all TIP30 primary antibodies
DescriptionRabbit monoclonal [EPR13185] to TIP30
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TIP30 aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9BUP3
- Human fetal liver tissue lysate; HeLa, A431, A549 and SW480 cell lysates
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181378 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 27 kDa.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionOxidoreductase required for tumor suppression. NAPDH-bound form inhibits nuclear import by competing with nuclear import substrates for binding to a subset of nuclear transport receptors. May act as a redox sensor linked to transcription through regulation of nuclear import. Isoform 1 is a metastasis suppressor with proapoptotic as well as antiangiogenic properties. Isoform 2 has an antiapoptotic effect.
Tissue specificityUbiquitous. Highest level in liver. High levels in lung, skeletal muscle, pancreas and placenta. Moderate levels in heart and kidney. Low levels in brain. Not expressed or low levels in variant small cell lung carcinomas, 33% of hepatocellular carcinomas and neuroblastomas.
DomainUnique C-terminus confers high proteasome-dependent instability to isoform 2.
Cellular localizationCytoplasm. Nucleus envelope.
- Information by UniProt
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All lanes : Anti-TIP30 antibody [EPR13185] (ab181378) at 1/10000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : HeLa cell lysate
Lane 3 : A431 cell lysate
Lane 4 : A549 cell lysate
Lane 5 : SW480 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 27 kDa
Western blot analysis on immunoprecipitation pellet from A431 cell lysate labeling TIP30 with ab181378 at 1/10 dilution (Lane 1). Lane 2 is 1XPBS (negative control). Detection with HRP-conjugated anti-rabbit IgG, detecting the non-reduced form of rabbit IgG.
Flow cytometric analysis of permeabilized HeLa cells (red) labeling TIP30 with ab181378 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).
ab181378 has not yet been referenced specifically in any publications.