Overview

  • Product name

  • Description

    Rabbit polyclonal to TIP49A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Horse, Cow, Dog, Drosophila melanogaster, Zebrafish, Gorilla
  • Immunogen

    Synthetic peptide within Human TIP49A aa 200-250. The exact sequence is proprietary. (NP_003698.1).
    Database link: Q9Y265

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab226001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 50 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex.
    May be able to bind plasminogen at cell surface and enhance plasminogen activation.
    Essential for cell proliferation.
  • Tissue specificity

    Ubiquitously expressed with high expression in heart, skeletal muscle and testis.
  • Sequence similarities

    Belongs to the ruvB family.
  • Domain

    Binding to MYC is dependent on a Myc domain essential for oncogenic activity.
  • Cellular localization

    Nucleus matrix. Nucleus > nucleoplasm. Cytoplasm. Membrane. Cytoplasm > cytoskeleton > centrosome. Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol, although it is also present in the cytoplasm and associated with the cell membranes. In prophase and prometaphase it is located at the centrosome and the branching microtubule spindles. After mitotic nuclear membrane disintigration it accumulates at the centrosome and sites of tubulin polymerization. As cells pass through metaphase and into telophase it is located close to the centrosome at the early phase of tubulin polymerization. In anaphase it accumulates at the zone of tubule interdigitation. In telophase it is found at polar tubule overlap, and it reappears at the site of chromosomal decondensation in the daughter cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • 49 kDa TATA box binding protein interacting protein antibody
    • 49 kDa TATA box-binding protein-interacting protein antibody
    • 49 kDa TBP interacting protein antibody
    • 49 kDa TBP-interacting protein antibody
    • 54 kDa erythrocyte cytosolic protein antibody
    • ECP-54 antibody
    • ECP54 antibody
    • ERYTHROCYTE CYTOSOLIC PROTEIN, 54-KD antibody
    • INO80 complex subunit H antibody
    • NMP 238 antibody
    • Nuclear matrix protein 238 antibody
    • Pontin 52 antibody
    • PONTIN antibody
    • RuvB like 1 antibody
    • RuvB like AAA ATPase 1 antibody
    • RuvB-like 1 antibody
    • RUVB1_HUMAN antibody
    • RUVBL1 antibody
    • RVB1 antibody
    • TAP54 alpha antibody
    • TAP54-alpha antibody
    • TIP49 antibody
    • TIP49a antibody
    • TIP60 associated protein 54 alpha antibody
    • TIP60-associated protein 54-alpha antibody
    see all

Images

  • All lanes : Anti-TIP49A antibody (ab226001) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 50 kDa


    Exposure time: 30 seconds


    Lysates prepared using NETN lysis buffer.

  • TIP49A was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226001 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226001 at 0.4 µg/ml.

    Lane 1: ab226001 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab226001 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Arabidopsis thaliana Tissue lysate - whole (leaf)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
1 µg
Specification
leaf
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. David Alabadi

Verified customer

Submitted May 22 2019

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