• Product name
  • Description
    Chicken polyclonal to TIP49A
  • Host species
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 2-280 of Human TIP49A



Our Abpromise guarantee covers the use of ab37754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    WB: 1/2000. Detects a band of approximately 80 kDa for the fusion protein (predicted molecular weight for the native protein: 53 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex.
      May be able to bind plasminogen at cell surface and enhance plasminogen activation.
      Essential for cell proliferation.
    • Tissue specificity
      Ubiquitously expressed with high expression in heart, skeletal muscle and testis.
    • Sequence similarities
      Belongs to the ruvB family.
    • Domain
      Binding to MYC is dependent on a Myc domain essential for oncogenic activity.
    • Cellular localization
      Nucleus matrix. Nucleus > nucleoplasm. Cytoplasm. Membrane. Cytoplasm > cytoskeleton > centrosome. Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol, although it is also present in the cytoplasm and associated with the cell membranes. In prophase and prometaphase it is located at the centrosome and the branching microtubule spindles. After mitotic nuclear membrane disintigration it accumulates at the centrosome and sites of tubulin polymerization. As cells pass through metaphase and into telophase it is located close to the centrosome at the early phase of tubulin polymerization. In anaphase it accumulates at the zone of tubule interdigitation. In telophase it is found at polar tubule overlap, and it reappears at the site of chromosomal decondensation in the daughter cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • 49 kDa TATA box binding protein interacting protein antibody
      • 49 kDa TATA box-binding protein-interacting protein antibody
      • 49 kDa TBP interacting protein antibody
      • 49 kDa TBP-interacting protein antibody
      • 54 kDa erythrocyte cytosolic protein antibody
      • ECP-54 antibody
      • ECP54 antibody
      • INO80 complex subunit H antibody
      • NMP 238 antibody
      • Nuclear matrix protein 238 antibody
      • Pontin 52 antibody
      • PONTIN antibody
      • RuvB like 1 antibody
      • RuvB like AAA ATPase 1 antibody
      • RuvB-like 1 antibody
      • RUVB1_HUMAN antibody
      • RUVBL1 antibody
      • RVB1 antibody
      • TAP54 alpha antibody
      • TAP54-alpha antibody
      • TIP49 antibody
      • TIP49a antibody
      • TIP60 associated protein 54 alpha antibody
      • TIP60-associated protein 54-alpha antibody
      see all


    • Anti-TIP49A antibody (ab37754) at 1/2000 dilution + E coli derived fusion protein at 2.5 µg

      Goat anti IgY HRP at 1/1000 dilution

      Predicted band size: 53 kDa
      Observed band size: 80 kDa (why is the actual band size different from the predicted?)

      The arrow next to the image points to the TIP49A fusion protein.
      Observed band size may differ from predicted the band size because the test antigen is a fusion protein.
      A colorimetric method was used for signal development.


    ab37754 has not yet been referenced specifically in any publications.

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