Overview

  • Product name

    Anti-TIP49A antibody [EPR6028]
    See all TIP49A primary antibodies
  • Description

    Rabbit monoclonal [EPR6028] to TIP49A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to amino acid residues near the C-teminal of Human TIP49A (UniProt ID: Q9Y265)

  • Positive control

    • K562, HT1080 and HT29 cell lysates. 293T cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex.
      May be able to bind plasminogen at cell surface and enhance plasminogen activation.
      Essential for cell proliferation.
    • Tissue specificity

      Ubiquitously expressed with high expression in heart, skeletal muscle and testis.
    • Sequence similarities

      Belongs to the ruvB family.
    • Domain

      Binding to MYC is dependent on a Myc domain essential for oncogenic activity.
    • Cellular localization

      Nucleus matrix. Nucleus > nucleoplasm. Cytoplasm. Membrane. Cytoplasm > cytoskeleton > centrosome. Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol, although it is also present in the cytoplasm and associated with the cell membranes. In prophase and prometaphase it is located at the centrosome and the branching microtubule spindles. After mitotic nuclear membrane disintigration it accumulates at the centrosome and sites of tubulin polymerization. As cells pass through metaphase and into telophase it is located close to the centrosome at the early phase of tubulin polymerization. In anaphase it accumulates at the zone of tubule interdigitation. In telophase it is found at polar tubule overlap, and it reappears at the site of chromosomal decondensation in the daughter cells.
    • Information by UniProt
    • Database links

    • Alternative names

      • 49 kDa TATA box binding protein interacting protein antibody
      • 49 kDa TATA box-binding protein-interacting protein antibody
      • 49 kDa TBP interacting protein antibody
      • 49 kDa TBP-interacting protein antibody
      • 54 kDa erythrocyte cytosolic protein antibody
      • ECP-54 antibody
      • ECP54 antibody
      • ERYTHROCYTE CYTOSOLIC PROTEIN, 54-KD antibody
      • INO80 complex subunit H antibody
      • NMP 238 antibody
      • Nuclear matrix protein 238 antibody
      • Pontin 52 antibody
      • PONTIN antibody
      • RuvB like 1 antibody
      • RuvB like AAA ATPase 1 antibody
      • RuvB-like 1 antibody
      • RUVB1_HUMAN antibody
      • RUVBL1 antibody
      • RVB1 antibody
      • TAP54 alpha antibody
      • TAP54-alpha antibody
      • TIP49 antibody
      • TIP49a antibody
      • TIP60 associated protein 54 alpha antibody
      • TIP60-associated protein 54-alpha antibody
      see all

    Images

    • All lanes : Anti-TIP49A antibody [EPR6028] (ab133513) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HT1080 cell lysate
      Lane 4 : HT29 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 50 kDa

    • Flow cytometric analysis of permeablized 293T cells labelling TIP49A with ab133513 at 1/10 dilution (red) or negative Rabbit IgG (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • McClurg UL  et al. Human ex vivo prostate tissue model system identifies ING3 as an oncoprotein. Br J Cancer 118:713-726 (2018). Read more (PubMed: 29381681) »
    • O'Neill DJ  et al. SETD6 controls the expression of estrogen-responsive genes and proliferation of breast carcinoma cells. Epigenetics 9:942-50 (2014). Read more (PubMed: 24751716) »
    See all 2 Publications for this product

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