Overview

  • Product name

  • Description

    Rabbit polyclonal to TIPE2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human TIPE2 (internal sequence).

  • Positive control

    • WB: Extracts from HuvEc cells.
  • General notes

    Protein previously known as Tumor necrosis factor alpha-induced protein 8-like protein 2.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.88% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab110389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 21 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or using a pressure cooker for about 5 minutes.

Target

  • Function

    Acts as a negative regulator of innate and adaptive immunity by maintaining immune homeostasis. Negative regulator of Toll-like receptor and T-cell receptor function. Prevents hyperresponsiveness of the immune system and maintains immune homeostasis. Inhibits JUN/AP1 and NF-kappa-B activation. Promotes Fas-induced apoptosis.
  • Sequence similarities

    Belongs to the TNFAIP8 family. TNFAIP8L2 subfamily.
  • Domain

    The central region was initially thought to constitute a DED (death effector) domain. However, 3D-structure data reveal a previously uncharacterized fold that is different from the predicted fold of a DED (death effector) domain. It consists of a large, hydrophobic central cavity that is poised for cofactor binding.
  • Information by UniProt
  • Database links

  • Alternative names

    • AW610835 antibody
    • FLJ23467 antibody
    • Inflammation factor 20 antibody
    • Inflammation factor protein 20 antibody
    • TIPE2 antibody
    • TNF alpha-induced protein 8-like protein 2 antibody
    • TNFAIP8-like protein 2 antibody
    • TNFAIP8L2 antibody
    • TP8L2_HUMAN antibody
    • Tumor necrosis factor alpha induced protein 8 like 2 antibody
    • Tumor necrosis factor alpha-induced protein 8-like protein 2 antibody
    see all

Images

  • All lanes : Anti-TIPE2 antibody (ab110389) at 1/500 dilution

    Lane 1 : Extracts from HuvEc cells
    Lane 2 : Extracts from HuvEc cells with blocking peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 21 kDa

References

This product has been referenced in:

  • Zhu L  et al. TIPE2 suppresses progression and tumorigenesis of esophageal carcinoma via inhibition of the Wnt/ß-catenin pathway. J Transl Med 16:7 (2018). Read more (PubMed: 29343267) »
  • Jiang Y  et al. TIPE2 governs macrophage polarization via negative regulation of mTORC1. Mol Med Rep 17:952-960 (2018). Read more (PubMed: 29115630) »
See all 3 Publications for this product

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