Anti-TIPIN antibody (ab229329)
Key features and details
- Rabbit polyclonal to TIPIN
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TIPIN antibody
See all TIPIN primary antibodies -
Description
Rabbit polyclonal to TIPIN -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow -
Positive control
- WB: TIPIN-transfected HEK-293T whole cell lysate; HEK-293T, A431, HeLa and HepG2 whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229329 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa. |
Target
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Function
Required for normal progression of S-phase. Important for cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. -
Sequence similarities
Belongs to the CSM3 family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 506176 Cow
- Entrez Gene: 54962 Human
- Omim: 610716 Human
- SwissProt: Q9BVW5 Human
- Unigene: 740671 Human
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Alternative names
- FLJ20516 antibody
- TIMELESS interacting protein antibody
- TIMELESS-interacting protein antibody
see all
Images
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All lanes : Anti-TIPIN antibody (ab229329) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : TIPIN-transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 35 kDa12 % SDS-PAGE gel.
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All lanes : Anti-TIPIN antibody (ab229329) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 35 kDa10 % SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab229329 has been referenced in 1 publication.
- Aricthota S & Haldar D DDK/Hsk1 phosphorylates and targets fission yeast histone deacetylase Hst4 for degradation to stabilize stalled DNA replication forks. Elife 10:N/A (2021). PubMed: 34608864