Key features and details
- Rabbit polyclonal to TIPIN - N-terminal
- Suitable for: WB, IP, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TIPIN antibody - N-terminal
See all TIPIN primary antibodies
DescriptionRabbit polyclonal to TIPIN - N-terminal
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human TIPIN (N terminal). ~300 aa.
Database link: Q9BVW5
- WB: U-2 OS and NIH/3T3 cell lysate. IP: U-2 OS cell lysate. ICC/IF: U-2 OS cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab236451 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 35 kDa.|
FunctionRequired for normal progression of S-phase. Important for cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light.
Sequence similaritiesBelongs to the CSM3 family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- FLJ20516 antibody
- TIMELESS interacting protein antibody
- TIMELESS-interacting protein antibody
All lanes : Anti-TIPIN antibody - N-terminal (ab236451) at 1/2000 dilution
Lane 1 : U-2 OS (Human bone osteosarcoma epithelial cell line) treated with control siRNA cell lysate
Lane 2 : U-2 OS (Human bone osteosarcoma epithelial cell line) treated with TIPIN siRNA cell lysate
Lane 3 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 35 kDa
Blocking: 5% TBS-T milk.
Incubated with primary antibody overnight.
2% paraformaldehyde-fixed U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling TIPIN with ab236451 (green) at 1/600 dilution in ICC/IF. Cells were treated with siRNA against TIPIN (right panel) or control (left panel). Counter stained with DAPI (blue). Incubated with primary antibody overnight at +4°C.
TIPIN was immunoprecipitated from U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate using 3 µl of ab236451.
Lane 1: U-2 OS whole cell lysate ~400 µg (Input).
Lane 2: Control IgG in U-2 OS whole cell lysate.
Lane 3: ab236451 in U-2 OS whole cell lysate.
For western blotting ab236451 was used at a 1/2000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236451 has not yet been referenced specifically in any publications.