Overview

  • Product name

    Anti-TIPIN antibody - N-terminal
    See all TIPIN primary antibodies
  • Description

    Rabbit polyclonal to TIPIN - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human TIPIN (N terminal). ~300 aa.
    Database link: Q9BVW5

  • Positive control

    • WB: U-2 OS and NIH/3T3 cell lysate. IP: U-2 OS cell lysate. ICC/IF: U-2 OS cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab236451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 35 kDa.
IP 1/2000.
ICC/IF 1/600.

Target

  • Function

    Required for normal progression of S-phase. Important for cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light.
  • Sequence similarities

    Belongs to the CSM3 family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ20516 antibody
    • TIMELESS interacting protein antibody
    • TIMELESS-interacting protein antibody
    • TIPIN antibody
    • TIPIN_HUMAN antibody
    see all

Images

  • All lanes : Anti-TIPIN antibody - N-terminal (ab236451) at 1/2000 dilution

    Lane 1 : U-2 OS (Human bone osteosarcoma epithelial cell line) treated with control siRNA cell lysate
    Lane 2 : U-2 OS (Human bone osteosarcoma epithelial cell line) treated with TIPIN siRNA cell lysate
    Lane 3 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 35 kDa



    Blocking: 5% TBS-T milk.

    Incubated with primary antibody overnight.

  • 2% paraformaldehyde-fixed U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling TIPIN with ab236451 (green) at 1/600 dilution in ICC/IF. Cells were treated with siRNA against TIPIN (right panel) or control (left panel). Counter stained with DAPI (blue). Incubated with primary antibody overnight at +4°C.

  • TIPIN was immunoprecipitated from U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate using 3 µl of ab236451.

    Lane 1: U-2 OS whole cell lysate ~400 µg (Input).

    Lane 2: Control IgG in U-2 OS whole cell lysate.

    Lane 3: ab236451 in U-2 OS whole cell lysate.

    For western blotting ab236451 was used at a 1/2000 dilution.

References

ab236451 has not yet been referenced specifically in any publications.

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