Product nameAnti-TIPIN antibody - N-terminal
See all TIPIN primary antibodies
DescriptionRabbit polyclonal to TIPIN - N-terminal
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human TIPIN (N terminal). ~300 aa.
Database link: Q9BVW5
- WB: U-2 OS and NIH/3T3 cell lysate. IP: U-2 OS cell lysate. ICC/IF: U-2 OS cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Our Abpromise guarantee covers the use of ab236451 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 35 kDa.|
FunctionRequired for normal progression of S-phase. Important for cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light.
Sequence similaritiesBelongs to the CSM3 family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- FLJ20516 antibody
- TIMELESS interacting protein antibody
- TIMELESS-interacting protein antibody
All lanes : Anti-TIPIN antibody - N-terminal (ab236451) at 1/2000 dilution
Lane 1 : U-2 OS (Human bone osteosarcoma epithelial cell line) treated with control siRNA cell lysate
Lane 2 : U-2 OS (Human bone osteosarcoma epithelial cell line) treated with TIPIN siRNA cell lysate
Lane 3 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 35 kDa
Blocking: 5% TBS-T milk.
Incubated with primary antibody overnight.
2% paraformaldehyde-fixed U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling TIPIN with ab236451 (green) at 1/600 dilution in ICC/IF. Cells were treated with siRNA against TIPIN (right panel) or control (left panel). Counter stained with DAPI (blue). Incubated with primary antibody overnight at +4°C.
TIPIN was immunoprecipitated from U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate using 3 µl of ab236451.
Lane 1: U-2 OS whole cell lysate ~400 µg (Input).
Lane 2: Control IgG in U-2 OS whole cell lysate.
Lane 3: ab236451 in U-2 OS whole cell lysate.
For western blotting ab236451 was used at a 1/2000 dilution.
ab236451 has not yet been referenced specifically in any publications.