Product nameAnti-TIPRL antibody
See all TIPRL primary antibodies
DescriptionRabbit polyclonal to TIPRL
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residues 200 and 250 of human TIPRL (NP_690866.1)
- HeLa, 293T and NIH3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab71974 was affinity purified using an epitope specific to TIPRL immobilized on solid support.
Our Abpromise guarantee covers the use of ab71974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 32 kDa).|
FunctionMay be a allosteric regulator of serine/threonine-protein phosphatase 2A (PP2A). Isoform 1 inhibits catalytic activity of the PP2A(D) core complex in vitro. The PP2A(C):TIPRL complex does not show phosphatase activity. May play a role in the regulation of ATM/ATR signaling pathway controlling DNA replication and repair.
Sequence similaritiesBelongs to the TIP41 family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- 1810011K17Rik antibody
- dJ69E11.3 antibody
- MGC3794 antibody
All lanes : Anti-TIPRL antibody (ab71974) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 32 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
ab71974 has not yet been referenced specifically in any publications.