Anti-TIRAP antibody (ab17218)
Key features and details
- Rabbit polyclonal to TIRAP
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TIRAP antibody
See all TIRAP primary antibodies -
Description
Rabbit polyclonal to TIRAP -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rhesus monkey -
Immunogen
Synthetic peptide. 15 amino acids near the C-terminus of mouse TIRAP (GenBank accession no.AAL05628).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab17218 is purified by ion exchange chromatography. TIRAP Antibody is affinity chromatography purified via peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 34 kDa.
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ICC/IF | (1) |
Use a concentration of 10 µg/ml.
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IHC-P | (1) |
Use a concentration of 2 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 34 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
IHC-P
Use a concentration of 2 µg/ml. |
Target
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Relevance
The Toll-like receptor (TLR) family in mammals comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TIRAP (TIR domain-containing adaptor protein) is an adaptor protein used by TLR4. Blocking TIRAP inhibits TLR4-mediated signaling events, including DC maturation and cytokine production. Function: Adapter involved in TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha and interleukin-6. Tissue specificity: Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain. Post-translational modification: Phosphorylated by IRAK1 and IRAK4. Also phosphorylated by BTK. Sequence similarities: Contains 1 TIR domain. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 114609 Human
- Entrez Gene: 117149 Mouse
- Entrez Gene: 680127 Rat
- GenBank: AF378129 Human
- GenBank: AF378130 Human
- Omim: 606252 Human
- SwissProt: P58753 Human
- SwissProt: Q99JY1 Mouse
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Alternative names
- Adapter protein wyatt antibody
- FLJ42305 antibody
- MAL antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab17218 has been referenced in 13 publications.
- Scopelliti F et al. The phosphoinositide-3-kinase (PI3K)-delta inhibitor seletalisib impairs monocyte-derived dendritic cells maturation, APC function, and promotes their migration to CCR7 and CXCR4 ligands. J Leukoc Biol 112:383-393 (2022). PubMed: 35199885
- Zhao G et al. Toll-like receptor 2 signaling pathway activation contributes to a highly efficient inflammatory response in Japanese encephalitis virus-infected mouse microglial cells by proteomics. Front Microbiol 13:989183 (2022). PubMed: 36171749
- Shen L et al. A naturally derived small molecule NDSM253 inhibits IKK1 to suppress inflammation response and promote bone healing after fracture. Am J Transl Res 13:24-37 (2021). PubMed: 33527006
- Hu X et al. Toll-like receptor 4 is a master regulator for colorectal cancer growth under high-fat diet by programming cancer metabolism. Cell Death Dis 12:791 (2021). PubMed: 34385421
- Choltus H et al. Cigarette Smoke Condensate Exposure Induces Receptor for Advanced Glycation End-Products (RAGE)-Dependent Sterile Inflammation in Amniotic Epithelial Cells. Int J Mol Sci 22:N/A (2021). PubMed: 34361111