Anti-TIRAP antibody (ab17218)
- Datasheet
- References (6)
- Protocols
Overview
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Product name
Anti-TIRAP antibody
See all TIRAP primary antibodies -
Description
Rabbit polyclonal to TIRAP -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rhesus monkey
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Immunogen
Synthetic peptide. 15 amino acids near the C-terminus of mouse TIRAP (GenBank accession no.AAL05628).
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Positive control
- Human heart cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab17218 is purified by ion exchange chromatography. TIRAP Antibody is affinity chromatography purified via peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab17218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 34 kDa. | |
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 2 µg/ml. |
Target
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Relevance
The Toll-like receptor (TLR) family in mammals comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TIRAP (TIR domain-containing adaptor protein) is an adaptor protein used by TLR4. Blocking TIRAP inhibits TLR4-mediated signaling events, including DC maturation and cytokine production. Function: Adapter involved in TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha and interleukin-6. Tissue specificity: Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain. Post-translational modification: Phosphorylated by IRAK1 and IRAK4. Also phosphorylated by BTK. Sequence similarities: Contains 1 TIR domain. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 114609 Human
- Entrez Gene: 117149 Mouse
- Entrez Gene: 680127 Rat
- GenBank: AF378129 Human
- GenBank: AF378130 Human
- Omim: 606252 Human
- SwissProt: P58753 Human
- SwissProt: Q99JY1 Mouse
see all -
Alternative names
- Adapter protein wyatt antibody
- FLJ42305 antibody
- MAL antibody
see all
Images
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Western blot - Anti-TIRAP antibody (ab17218)Western Blot of human heart lysate labeling TIRAP with Anti-TIRAP antibody (ab17218) at (1) 1 and (2) 2µg/ml. -
Western blot - Anti-TIRAP antibody (ab17218)Lane 1 : Anti-TIRAP antibody (ab17218) at 0.5 µg/ml
Lane 2 : Anti-TIRAP antibody (ab17218) at 1 µg/ml
Lane 3 : Anti-TIRAP antibody (ab17218) at 2 µg/ml
All lanes : heart cell lysate
Observed band size: 35 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIRAP antibody (ab17218)ab17218 at 2µg/ml staining TIRAP in human heart cells by IHC
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Immunocytochemistry/ Immunofluorescence - Anti-TIRAP antibody (ab17218)This image is courtesy of an Anonymous Abreview.ab17218 staining TIRAP in a PMA-treated Human THP-1 cell by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde for 30 minutes and permeabilized in 0.1% Triton X-100 prior to blocking in 5% serum for 1 hour at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 20°C. The secondary antibody was Alexa Fluor® 488-conjugated goat anti-rabbit polyclonal, diluted 1/500 (green). Other stains used were Alexa Fluor 595-conjugated wheat germ agglutinin (red) and Nuclei were stained with Hoechst 33258 (blue)
Protocols
Datasheets and documents
References
This product has been referenced in:
- Kapelouzou A et al. Differential expression patterns of Toll Like Receptors and Interleukin-37 between calcific aortic and mitral valve cusps in humans. Cytokine 116:150-160 (2019). Read more (PubMed: 30716659) »
- Jakka P et al. Cytoplasmic Linker Protein CLIP170 Negatively Regulates TLR4 Signaling by Targeting the TLR Adaptor Protein TIRAP. J Immunol 200:704-714 (2018). Read more (PubMed: 29222167) »
