Anti-TIRAP antibody (ab17218)
Key features and details
- Rabbit polyclonal to TIRAP
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TIRAP antibody
See all TIRAP primary antibodies -
Description
Rabbit polyclonal to TIRAP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rhesus monkey
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Immunogen
Synthetic peptide. 15 amino acids near the C-terminus of mouse TIRAP (GenBank accession no.AAL05628).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab17218 is purified by ion exchange chromatography. TIRAP Antibody is affinity chromatography purified via peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 34 kDa.
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| IHC-P | (1) |
Use a concentration of 2 µg/ml.
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| Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 34 kDa. |
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IHC-P
Use a concentration of 2 µg/ml. |
Target
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Relevance
The Toll-like receptor (TLR) family in mammals comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TIRAP (TIR domain-containing adaptor protein) is an adaptor protein used by TLR4. Blocking TIRAP inhibits TLR4-mediated signaling events, including DC maturation and cytokine production. Function: Adapter involved in TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha and interleukin-6. Tissue specificity: Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain. Post-translational modification: Phosphorylated by IRAK1 and IRAK4. Also phosphorylated by BTK. Sequence similarities: Contains 1 TIR domain. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 114609 Human
- Entrez Gene: 117149 Mouse
- Entrez Gene: 680127 Rat
- GenBank: AF378129 Human
- GenBank: AF378130 Human
- Omim: 606252 Human
- SwissProt: P58753 Human
- SwissProt: Q99JY1 Mouse
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Alternative names
- Adapter protein wyatt antibody
- FLJ42305 antibody
- MAL antibody
see all
Images
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Western blot - Anti-TIRAP antibody (ab17218)Western Blot of human heart lysate labeling TIRAP with Anti-TIRAP antibody (ab17218) at (1) 1 and (2) 2µg/ml.
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Western blot - Anti-TIRAP antibody (ab17218)Lane 1 : Anti-TIRAP antibody (ab17218) at 0.5 µg/ml
Lane 2 : Anti-TIRAP antibody (ab17218) at 1 µg/ml
Lane 3 : Anti-TIRAP antibody (ab17218) at 2 µg/ml
All lanes : heart cell lysate
Observed band size: 35 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIRAP antibody (ab17218)ab17218 at 2µg/ml staining TIRAP in human heart cells by IHC
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab17218 has been referenced in 7 publications.
- Liu L et al. BIG1 controls macrophage pro-inflammatory responses through ARF3-mediated PI(4,5)P2 synthesis. Cell Death Dis 11:374 (2020). PubMed: 32415087
- Kapelouzou A et al. Differential expression patterns of Toll Like Receptors and Interleukin-37 between calcific aortic and mitral valve cusps in humans. Cytokine 116:150-160 (2019). PubMed: 30716659
- Jakka P et al. Cytoplasmic Linker Protein CLIP170 Negatively Regulates TLR4 Signaling by Targeting the TLR Adaptor Protein TIRAP. J Immunol 200:704-714 (2018). PubMed: 29222167
- Sakuma C et al. Specific binding of the WASP N-terminal domain to Btk is critical for TLR2 signaling in macrophages. Mol Immunol 63:328-36 (2015). PubMed: 25213142
- Sakaguchi M et al. DNAX-activating protein 10 (DAP10) membrane adaptor associates with receptor for advanced glycation end products (RAGE) and modulates the RAGE-triggered signaling pathway in human keratinocytes. J Biol Chem 289:23389-402 (2014). WB ; Human . PubMed: 25002577
- Sakuma C et al. Critical roles of the WASP N-terminal domain and Btk in LPS-induced inflammatory response in macrophages. PLoS One 7:e30351 (2012). WB ; Mouse . PubMed: 22253930
- Sakaguchi M et al. TIRAP, an adaptor protein for TLR2/4, transduces a signal from RAGE phosphorylated upon ligand binding. PLoS One 6:e23132 (2011). WB . PubMed: 21829704