Anti-TIRAP antibody (ab17218)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameAnti-TIRAP antibody
See all TIRAP primary antibodies -
DescriptionRabbit polyclonal to TIRAP
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rhesus monkey
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Immunogen
Synthetic peptide. 15 amino acids near the C-terminus of mouse TIRAP (GenBank accession no.AAL05628).
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Positive control
- Human heart cell lysates
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
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Storage bufferPreservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab17218 is purified by ion exchange chromatography. TIRAP Antibody is affinity chromatography purified via peptide column.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab17218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 34 kDa.Can be blocked with Mouse TIRAP peptide (ab39884). | |
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 2 µg/ml. |
Target
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RelevanceThe Toll-like receptor (TLR) family in mammals comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TIRAP (TIR domain-containing adaptor protein) is an adaptor protein used by TLR4. Blocking TIRAP inhibits TLR4-mediated signaling events, including DC maturation and cytokine production. Function: Adapter involved in TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha and interleukin-6. Tissue specificity: Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain. Post-translational modification: Phosphorylated by IRAK1 and IRAK4. Also phosphorylated by BTK. Sequence similarities: Contains 1 TIR domain.
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Cellular localizationCytoplasmic
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Database links
- Entrez Gene: 114609 Human
- Entrez Gene: 117149 Mouse
- Entrez Gene: 680127 Rat
- GenBank: AF378129 Human
- GenBank: AF378130 Human
- Omim: 606252 Human
- SwissProt: P58753 Human
- SwissProt: Q99JY1 Mouse
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Alternative names
- Adapter protein wyatt antibody
- FLJ42305 antibody
- MAL antibody
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Images
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Western blot - Anti-TIRAP antibody (ab17218)Western Blot of human heart lysate labeling TIRAP with Anti-TIRAP antibody (ab17218) at (1) 1 and (2) 2µg/ml. -
Western blot - Anti-TIRAP antibody (ab17218)Lane 1 : Anti-TIRAP antibody (ab17218) at 0.5 µg/ml
Lane 2 : Anti-TIRAP antibody (ab17218) at 1 µg/ml
Lane 3 : Anti-TIRAP antibody (ab17218) at 2 µg/ml
All lanes : heart cell lysate
Observed band size: 35 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIRAP antibody (ab17218)ab17218 at 2µg/ml staining TIRAP in human heart cells by IHC
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Immunocytochemistry/ Immunofluorescence - Anti-TIRAP antibody (ab17218)This image is courtesy of an Anonymous Abreview.ab17218 staining TIRAP in a PMA-treated Human THP-1 cell by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde for 30 minutes and permeabilized in 0.1% Triton X-100 prior to blocking in 5% serum for 1 hour at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 20°C. The secondary antibody was Alexa Fluor® 488-conjugated goat anti-rabbit polyclonal, diluted 1/500 (green). Other stains used were Alexa Fluor 595-conjugated wheat germ agglutinin (red) and Nuclei were stained with Hoechst 33258 (blue)
Protocols
Datasheets and documents
References
This product has been referenced in:
- Sakuma C et al. Specific binding of the WASP N-terminal domain to Btk is critical for TLR2 signaling in macrophages. Mol Immunol 63:328-36 (2015). Read more (PubMed: 25213142) »
- Sakaguchi M et al. DNAX-activating protein 10 (DAP10) membrane adaptor associates with receptor for advanced glycation end products (RAGE) and modulates the RAGE-triggered signaling pathway in human keratinocytes. J Biol Chem 289:23389-402 (2014). WB ; Human . Read more (PubMed: 25002577) »
