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    tirap-antibody-ab17218.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-TIRAP antibody (ab17218)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (7)

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Western blot - Anti-TIRAP antibody (ab17218)
  • Western blot - Anti-TIRAP antibody (ab17218)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIRAP antibody (ab17218)

Key features and details

  • Rabbit polyclonal to TIRAP
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Protein
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Recombinant Human TIRAP protein (ab131663)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Primary
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Anti-BTK antibody (ab25971)

View more associated products

Overview

  • Product name

    Anti-TIRAP antibody
    See all TIRAP primary antibodies
  • Description

    Rabbit polyclonal to TIRAP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Synthetic peptide. 15 amino acids near the C-terminus of mouse TIRAP (GenBank accession no.AAL05628).

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab17218 is purified by ion exchange chromatography. TIRAP Antibody is affinity chromatography purified via peptide column.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Immunology
    • Innate Immunity
    • TLR Signaling

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human TIRAP protein (ab131663)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab17218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 34 kDa.
IHC-P (1)
Use a concentration of 2 µg/ml.
Notes
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 34 kDa.
IHC-P
Use a concentration of 2 µg/ml.

Target

  • Relevance

    The Toll-like receptor (TLR) family in mammals comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TIRAP (TIR domain-containing adaptor protein) is an adaptor protein used by TLR4. Blocking TIRAP inhibits TLR4-mediated signaling events, including DC maturation and cytokine production. Function: Adapter involved in TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha and interleukin-6. Tissue specificity: Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain. Post-translational modification: Phosphorylated by IRAK1 and IRAK4. Also phosphorylated by BTK. Sequence similarities: Contains 1 TIR domain.
  • Cellular localization

    Cytoplasmic
  • Database links

    • Entrez Gene: 114609 Human
    • Entrez Gene: 117149 Mouse
    • Entrez Gene: 680127 Rat
    • GenBank: AF378129 Human
    • GenBank: AF378130 Human
    • Omim: 606252 Human
    • SwissProt: P58753 Human
    • SwissProt: Q99JY1 Mouse
    • Alternative names

      • Adapter protein wyatt antibody
      • FLJ42305 antibody
      • MAL antibody
      • MyD88 adapter like protein antibody
      • TIR domain containing adapter protein antibody
      • Toll interleukin 1 receptor (TIR) domain containing adaptor protein antibody
      • Toll interleukin 1 receptor domain containing adapter protein antibody
      • Toll like receptor adaptor protein antibody
      • Wyatt antibody
      see all

    Images

    • Western blot - Anti-TIRAP antibody (ab17218)
      Western blot - Anti-TIRAP antibody (ab17218)
      Western Blot of human heart lysate labeling TIRAP with Anti-TIRAP antibody (ab17218) at (1) 1 and (2) 2µg/ml.
    • Western blot - Anti-TIRAP antibody (ab17218)
      Western blot - Anti-TIRAP antibody (ab17218)
      Lane 1 : Anti-TIRAP antibody (ab17218) at 0.5 µg/ml
      Lane 2 : Anti-TIRAP antibody (ab17218) at 1 µg/ml
      Lane 3 : Anti-TIRAP antibody (ab17218) at 2 µg/ml

      All lanes : heart cell lysate

      Observed band size: 35 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIRAP antibody (ab17218)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIRAP antibody (ab17218)

      ab17218 at 2µg/ml staining TIRAP in human heart cells by IHC

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (7)

    Publishing research using ab17218? Please let us know so that we can cite the reference in this datasheet.

    ab17218 has been referenced in 7 publications.

    • Liu L  et al. BIG1 controls macrophage pro-inflammatory responses through ARF3-mediated PI(4,5)P2 synthesis. Cell Death Dis 11:374 (2020). PubMed: 32415087
    • Kapelouzou A  et al. Differential expression patterns of Toll Like Receptors and Interleukin-37 between calcific aortic and mitral valve cusps in humans. Cytokine 116:150-160 (2019). PubMed: 30716659
    • Jakka P  et al. Cytoplasmic Linker Protein CLIP170 Negatively Regulates TLR4 Signaling by Targeting the TLR Adaptor Protein TIRAP. J Immunol 200:704-714 (2018). PubMed: 29222167
    • Sakuma C  et al. Specific binding of the WASP N-terminal domain to Btk is critical for TLR2 signaling in macrophages. Mol Immunol 63:328-36 (2015). PubMed: 25213142
    • Sakaguchi M  et al. DNAX-activating protein 10 (DAP10) membrane adaptor associates with receptor for advanced glycation end products (RAGE) and modulates the RAGE-triggered signaling pathway in human keratinocytes. J Biol Chem 289:23389-402 (2014). WB ; Human . PubMed: 25002577
    • Sakuma C  et al. Critical roles of the WASP N-terminal domain and Btk in LPS-induced inflammatory response in macrophages. PLoS One 7:e30351 (2012). WB ; Mouse . PubMed: 22253930
    • Sakaguchi M  et al. TIRAP, an adaptor protein for TLR2/4, transduces a signal from RAGE phosphorylated upon ligand binding. PLoS One 6:e23132 (2011). WB . PubMed: 21829704

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TIRAP antibody

    Excellent
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated
    Sample
    Rhesus monkey Tissue sections (Colon)
    Specification
    Colon
    Permeabilization
    Yes - 0.1% Triton-x-100
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 23 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TIRAP antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (THP-1)
    Specification
    THP-1
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton X100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 10 2011

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