Anti-TIS11D antibody (ab70775)

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Western blot - Anti-TIS11D antibody (ab70775)

    Key features and details

    • Rabbit polyclonal to TIS11D
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-TIS11D antibody

    • Description

      Rabbit polyclonal to TIS11D

    • Host species

      Rabbit

    • Specificity

      ab70775 detects endogenous levels of total TIS11D protein.

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse

    • Immunogen

      Synthetic peptide corresponding to Human TIS11D (internal sequence).
      Database link: P47974

    • Positive control

      • A549 cell extracts.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab70775 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 52 kDa).

    Target

    • Function

      mRNA-binding protein that plays a key role in self-renewal of erythroid cells in response to glucocorticoids. Specifically binds to the AU-rich element (ARE) in the 3'-UTR of target mRNAs, promoting their deadenylation and degradation (PubMed:14981510). Specifically expressed in burst-forming unit-erythroid (BFU-E) progenitors in response to glucocorticoids and acts as a negative regulator of erythroid cell differentiation: promotes self-renewal of erythroid cells by binding mRNAs that are induced or highly expressed during terminal erythroid differentiation and promotes their degradation, preventing erythroid cell differentiation. Down-regulated during erythroid differentiation from the BFU-E stage, stabilizing mRNAs required for terminal differentiation.

    • Involvement in disease

      Defects in ZFP36L2 may be a cause of leukemias. Frameshfits mutations disrupting ZFP36L2 have been found in a patient with acute myeloid leukemia (PubMed:21109922).

    • Sequence similarities

      Contains 2 C3H1-type zinc fingers.

    • Cellular localization

      Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm.
    • Information by UniProt

    • Database links

      • Alternative names

        • BRF2 antibody
        • Butyrate response factor 2 antibody
        • C3H1 type-like 2 antibody
        • EGF response factor 2 antibody
        • EGF-response factor 2 antibody
        • ERF 2 antibody
        • ERF-2 antibody
        • ERF2 antibody
        • Protein TIS11D antibody
        • RNF162C antibody
        • TIS11D antibody
        • TISD_HUMAN antibody
        • ZFP36 like 2 antibody
        • ZFP36 ring finger protein like 2 antibody
        • ZFP36-like 2 antibody
        • Zfp36l2 antibody
        • Zinc finger protein 36 antibody
        • Zinc finger protein 36, C3H type like 1 antibody
        • zinc finger protein 36, C3H type like 2 antibody
        • Zinc finger protein 36, C3H1 type like 2 antibody
        • Zinc finger protein, C3H type, 36 like 2 antibody
        see all

      Images

      • Western blot - Anti-TIS11D antibody (ab70775)
        Western blot - Anti-TIS11D antibody (ab70775)
        All lanes : Anti-TIS11D antibody (ab70775) at 1/500 dilution

        Lane 1 : A549 cell extract
        Lane 2 : A549 cell extract with immunising peptide at 10 µg

        Lysates/proteins at 30 µg per lane.

        Predicted band size: 52 kDa
        Observed band size: 42 kDa
        why is the actual band size different from the predicted?

      References (3)

      Publishing research using ab70775? Please let us know so that we can cite the reference in this datasheet.

      ab70775 has been referenced in 3 publications.

      • Salerno F  et al. Translational repression of pre-formed cytokine-encoding mRNA prevents chronic activation of memory T cells. Nat Immunol N/A:N/A (2018). PubMed: 29988089
      • Dumdie JN  et al. Chromatin Modification and Global Transcriptional Silencing in the Oocyte Mediated by the mRNA Decay Activator ZFP36L2. Dev Cell 44:392-402.e7 (2018). PubMed: 29408237
      • Zhang L  et al. ZFP36L2 is required for self-renewal of early burst-forming unit erythroid progenitors. Nature 499:92-6 (2013). WB, IP ; Mouse . PubMed: 23748442

      Customer reviews and Q&As

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      1-3 of 3 Abreviews or Q&A

      Question

      I have asked for "sample size" antibody, if you have them available. The "100 ug" is a full-size vial which would suffice for at least a dozen of WB applications. We just would like to see how the Ab worked and if they would distinguish the isoforms (ZFP36, L1 and L2) before buying the full size.

      Read More
      Answer

      Thank you for your reply.

      I have heard back from the lab regarding ab83579:


      Crossreactivity with TISIIB and TISIID isn't something we have tested in house but an immunogen search shows that both TISIIB and TISIID only have 4 residues of the immunogen in common so we would say it is very unlikely that ab83579 would detect these 2 proteins.
      We do not offer smaller samples sizes than our selling size (due to operational resons), but have a guarantee in place that covers the antibody for 6 months after the purchase for the use in the tested species and applications as listed on the datasheet (https://www.abcam.com/Abpromise).
      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
      Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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      Question

      cross reactivity with TIS11A, TIS11B and TIS11D determined?

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      Answer

      Thank you for contacting us.

      I have received the following information regading 2 of the antibodies. For ab83579, I am still waiting for more information.

      ab70775:
      The antibody was raised against human TIS11D. This antibody has been validated for Western blot using A549 cell extracts. This antibody has not been tested against TIS11A or TIS11B. Alignment of the antigen sequence with TIS11A (http://www.uniprot.org/uniprot/P2665) shows a 60% homology.
      Alignment of the antigen sequence with TIS11B (http://www.uniprot.org/uniprot/Q07352) shows a 66.7% homology.

      ab53747:
      We have not obtained data from endogenous protein and have not tested cross reactivity with TIS11A and TIS11D.
      However, we checked cross reactivity using “BLAST search” between immunogen sequence of ab53747 and TIS11A, TIS11D. And the results are the homology is for TIS11D: 76%, and for TIS11A: 68%. Crossreactivity might be possible for TIS11D.

      I will be in touch with more information regarding ab83579.

      I hope this information is already of help to you. Please do not hesitate to contact us if you need any more advice or information.

      Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

      Read More

      Question

      NInquiry: You have the wrong size for this protein --you note this but do not provide an explanation. Is the immunogen sequence in the region that is distinct from Tis11b --our antibody against Tis11b recognizes both --we need to confirm this

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      Answer

      Thank you for contacting us.





      The immunogen sequence used to create this antibody is from aa231-270 ofhuman TIS11D. This sequence contains a 15aa span which has a 70% homology with TIS11B. At this level, we do not consider ab70775 to be cross reactive with TIS11B.

      Western blot is smaller than the band predicted by sequence. However, migration is affected by other factors and so the actual band size observed may differ from that predicted. Common factors include...

      post-translational modification- e.g. phosphorylation, glycosylation etc which increases the size of the protein
      post-translation cleavage- e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases
      splice variants- alternative splicing may create different sized proteins from the same gene
      relative charge- the composition of amino acids (charged vs non-charged)



      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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