Key features and details
- Rabbit polyclonal to TIS11D
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TIS11D antibody
DescriptionRabbit polyclonal to TIS11D
Specificityab70775 detects endogenous levels of total TIS11D protein.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human TIS11D (internal sequence).
Database link: P47974
- A549 cell extracts.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab70775 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Our Abpromise guarantee covers the use of ab70775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 52 kDa).|
FunctionmRNA-binding protein that plays a key role in self-renewal of erythroid cells in response to glucocorticoids. Specifically binds to the AU-rich element (ARE) in the 3'-UTR of target mRNAs, promoting their deadenylation and degradation (PubMed:14981510). Specifically expressed in burst-forming unit-erythroid (BFU-E) progenitors in response to glucocorticoids and acts as a negative regulator of erythroid cell differentiation: promotes self-renewal of erythroid cells by binding mRNAs that are induced or highly expressed during terminal erythroid differentiation and promotes their degradation, preventing erythroid cell differentiation. Down-regulated during erythroid differentiation from the BFU-E stage, stabilizing mRNAs required for terminal differentiation.
Involvement in diseaseDefects in ZFP36L2 may be a cause of leukemias. Frameshfits mutations disrupting ZFP36L2 have been found in a patient with acute myeloid leukemia (PubMed:21109922).
Sequence similaritiesContains 2 C3H1-type zinc fingers.
Cellular localizationCytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm.
- Information by UniProt
- BRF2 antibody
- Butyrate response factor 2 antibody
- C3H1 type-like 2 antibody
All lanes : Anti-TIS11D antibody (ab70775) at 1/500 dilution
Lane 1 : A549 cell extract
Lane 2 : A549 cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 52 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
ab70775 has been referenced in 3 publications.
- Salerno F et al. Translational repression of pre-formed cytokine-encoding mRNA prevents chronic activation of memory T cells. Nat Immunol N/A:N/A (2018). PubMed: 29988089
- Dumdie JN et al. Chromatin Modification and Global Transcriptional Silencing in the Oocyte Mediated by the mRNA Decay Activator ZFP36L2. Dev Cell 44:392-402.e7 (2018). PubMed: 29408237
- Zhang L et al. ZFP36L2 is required for self-renewal of early burst-forming unit erythroid progenitors. Nature 499:92-6 (2013). WB, IP ; Mouse . PubMed: 23748442