Product nameTissue Factor Activity Assay Kit (Human, Colorimetric)
See all Tissue Factor kits
Sample typeUrine, Serum, Plasma, Cell culture extracts, Tissue
Assay typeEnzyme activity (quantitative)
Assay time1h 00m
Species reactivityReacts with: Human
Tissue Factor Activity Assay Kit (Human, Colorimetric) ab108906 is used to determine Human Tissue Factor activity in plasma, tissue and cell culture supernatants.
The tissue factor activity assay protocol measures the ability of lipoprotein TF/FVIIa to activate factor X (FX) to factor Xa. The amidolytic activity of the TF/FVIIa complex is quantitated by the amount of FXa produced using a highly specific FXa substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA at 405 nm is directly proportional to the TF enzymatic activity.
Tissue factor activity assay protocol summary:
- add reaction mix to wells
- add standards and samples
- incubate for 30 min
- add FXa substrate
- analyze with a microplate reader every 5 min for 25 min
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Previously called Tissue Factor Human Chromogenic Activity Assay Kit.
The transmembrane protein Tissue factor (TF) is the physiologic trigger of coagulation in normal hemostasis. Tissue factor binds and allosterically activates factor VII (FVII). The TF-FVIIa complex cleaves factor IX and X, leading to thrombin generation. Tissue factor markedly enhances the ability of FVIIa to cleave both macromolecule and small peptidyl substrates.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests Assay Diluent 1 x 20ml FXa Substrate 2 units Human FVII 1 unit Human FX 1 unit rh Tissue Factor Standard (lipoprotein) 1 unit Sample Diluent 1 x 11ml Sealing Tapes 3 units Tissue Factor Microplate (12 strips of 8 wells) 1 unit
FunctionInitiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Sequence similaritiesBelongs to the tissue factor family.
- Information by UniProt
- CD142 antigen
- Coagulation factor III
Colorimetric standard curve: mean of duplicates (+/- SD)
Standard curve showing concentration (pM) on X-axis and increase of O.D. per min on Y-axis (time frame: 20 min.)
Time course showing O.D.'s of one standard point and one sample (undiluted vs.diluted)
Tissue Factor measured in various biologicals showing average concentrations
This product has been referenced in:
- Schulz A et al. Protective vascular coagulation in response to bacterial infection of the kidney is regulated by bacterial lipid A and host CD147. Pathog Dis N/A:N/A (2018). Read more (PubMed: 30476069) »
- Watanabe Y et al. Indoleamine 2,3-dioxygenase 1 in coronary atherosclerotic plaque enhances tissue factor expression in activated macrophages. Res Pract Thromb Haemost 2:726-735 (2018). Read more (PubMed: 30349892) »