Overview

  • Product name
    Anti-Tissue Factor antibody
    See all Tissue Factor primary antibodies
  • Description
    Rabbit polyclonal to Tissue Factor
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 33 - 295 of Human Tissue Factor (BC011029).

  • Positive control
    • WB: Human fetal kidney, brain, skin and lung lysates IHC-P: Human fetal kidney tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab104513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/100 - 1/500.

Target

Images

  • All lanes : Anti-Tissue Factor antibody (ab104513) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Human fetal skin lysate
    Lane 4 : Human fetal lung lysate

    Predicted band size: 33 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?

  • ab104513, at a 1/100 dilution, staining Tissue Factor in formalin-fixed, paraffin-embedded Human fetal kidney tissue, showing membrane staining.

References

This product has been referenced in:
  • Cheng X  et al. Trimethylamine N-oxide promotes tissue factor expression and activity in vascular endothelial cells: A new link between trimethylamine N-oxide and atherosclerotic thrombosis. Thromb Res 177:110-116 (2019). Read more (PubMed: 30875490) »
  • Khemais-Benkhiat S  et al. Angiotensin II-induced redox-sensitive SGLT1 and 2 expression promotes high glucose-induced endothelial cell senescence. J Cell Mol Med N/A:N/A (2019). Read more (PubMed: 30929316) »
See all 12 Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Mouse Cell lysate - other (aortic vascular smooth muscle cells)
Specification
aortic vascular smooth muscle cells
Treatment
Indoxy sulfate 0.25-100 ug/ml
Blocking step
Milk as blocking agent for 45 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C

Dr. Moshe Shashar

Verified customer

Submitted Jul 18 2014

Application
Flow Cytometry
Sample
Rat Cell (Plasma Microparticles)
Specification
Plasma Microparticles
Preparation
Cell harvesting/tissue preparation method: Centrifuge whole blood 1,500xg 15 min, then centrifuge the supernatant again at 13,000xg 2 min, and retain supernatant
Sample buffer: Annexin Binding Buffer
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
MP samples without label, and sample with secondary antibody only

Abcam user community

Verified customer

Submitted Feb 23 2012

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