Product nameAnti-Tissue Factor antibody
See all Tissue Factor primary antibodies
DescriptionRabbit polyclonal to Tissue Factor
Tested applicationsSuitable for: EIA, RIA, IP, WB, ICC/IF, ELISAmore details
Species reactivityReacts with: Human, African green monkey
Sf21 expressed human Tissue Factor.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.50
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab48647 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|EIA||Use at an assay dependent concentration.|
|RIA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 33 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
FunctionInitiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Sequence similaritiesBelongs to the tissue factor family.
- Information by UniProt
- CD142 antibody
- CD142 antigen antibody
- Coagulation factor III (thromboplastin tissue factor) antibody
ab48647 staining Tissue Factor in Cos-7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/300) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
This product has been referenced in:
- Wen L et al. Cooperation Between the Inflammation and Coagulation Systems Promotes the Survival of Circulating Tumor Cells in Renal Cell Carcinoma Patients. Front Oncol 9:504 (2019). Read more (PubMed: 31263677) »
- Evans CA et al. Reduced keratin expression in colorectal neoplasia and associated fields is reversible by diet and resection. BMJ Open Gastroenterol 2:e000022 (2015). WB . Read more (PubMed: 26462274) »