Recombinant
RabMAb

Recombinant Anti-Tissue Factor antibody [EPR18160-175] (ab189483)

Overview

  • Product name

    Anti-Tissue Factor antibody [EPR18160-175]
    See all Tissue Factor primary antibodies
  • Description

    Rabbit monoclonal [EPR18160-175] to Tissue Factor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment within Mouse Tissue Factor aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P20352

  • Positive control

    • WB: Mouse skin and brain tissue lysate; NIH/3T3 whole cell lysate. IP: Mouse brain tissue lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab189483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/30.
WB 1/1000. Detects a band of approximately 33, 47 kDa (predicted molecular weight: 33 kDa).

Target

  • Function

    Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
  • Sequence similarities

    Belongs to the tissue factor family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD142 antibody
    • CD142 antigen antibody
    • Coagulation factor III (thromboplastin tissue factor) antibody
    • Coagulation factor III antibody
    • F3 antibody
    • FLJ17960 antibody
    • TF antibody
    • TF_HUMAN antibody
    • TFA antibody
    • Thromboplastin antibody
    • Tissue factor antibody
    see all

Images

  • All lanes : Anti-Tissue Factor antibody [EPR18160-175] (ab189483) at 1/1000 dilution

    Lane 1 : Mouse skin tissue lysate
    Lane 2 : Mouse brain tissue lysate
    Lanes 3-4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 33 kDa
    Observed band size: 33, 47 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution: 5% NFDM/TBST.

    Exposure: Lanes 1 and 3: 3 minutes; Lanes 2 and 4: 10 seconds.

    The observed molecular mass is consistent with the literature (PMID: 7992837).

  • Tissue Factor was immunoprecipitated from 0.35 mg of mouse brain lysate with ab189483 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab189483 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Mouse brain lysate 10 µg (Input). 

    Lane 2: ab189483 IP in mouse brain lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab1189483 in mouse brain lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

References

ab189483 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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