Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18160-175] to Tissue Factor
- Suitable for: IP, WB
- Reacts with: Mouse
Product nameAnti-Tissue Factor antibody [EPR18160-175]
See all Tissue Factor primary antibodies
DescriptionRabbit monoclonal [EPR18160-175] to Tissue Factor
Tested applicationsSuitable for: IP, WBmore details
Unsuitable for: ICC or IHC-P
Species reactivityReacts with: Mouse
Recombinant fragment within Mouse Tissue Factor aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P20352
- WB: Mouse skin and brain tissue lysate; NIH/3T3 whole cell lysate. IP: Mouse brain tissue lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab189483 in the following tested applications.
|WB||1/1000. Detects a band of approximately 33, 47 kDa (predicted molecular weight: 33 kDa).|
FunctionInitiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Sequence similaritiesBelongs to the tissue factor family.
- Information by UniProt
- CD142 antibody
- CD142 antigen antibody
- Coagulation factor III (thromboplastin tissue factor) antibody
All lanes : Anti-Tissue Factor antibody [EPR18160-175] (ab189483) at 1/1000 dilution
Lane 1 : Mouse skin tissue lysate
Lane 2 : Mouse brain tissue lysate
Lanes 3-4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33, 47 kDa why is the actual band size different from the predicted?
Blocking/Dilution: 5% NFDM/TBST.
Exposure: Lanes 1 and 3: 3 minutes; Lanes 2 and 4: 10 seconds.
The observed molecular mass is consistent with the literature (PMID: 7992837).
Tissue Factor was immunoprecipitated from 0.35 mg of mouse brain lysate with ab189483 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab189483 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse brain lysate 10 µg (Input).
Lane 2: ab189483 IP in mouse brain lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
ab189483 has not yet been referenced specifically in any publications.