Recombinant Anti-Tissue Factor antibody [EPR18160-175] - BSA and Azide free (ab227910)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18160-175] to Tissue Factor - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-Tissue Factor antibody [EPR18160-175] - BSA and Azide free
See all Tissue Factor primary antibodies -
Description
Rabbit monoclonal [EPR18160-175] to Tissue Factor - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse skin and brain tissue lysate; NIH/3T3 whole cell lysate.
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General notes
ab227910 is the carrier-free version of ab189483.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18160-175 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 33, 47 kDa (predicted molecular weight: 33 kDa).
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IP |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 33, 47 kDa (predicted molecular weight: 33 kDa). |
IP
Use at an assay dependent concentration. |
Target
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Function
Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. -
Sequence similarities
Belongs to the tissue factor family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 14066 Mouse
- SwissProt: P20352 Mouse
- Unigene: 273188 Mouse
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Alternative names
- CD142 antibody
- CD142 antigen antibody
- Coagulation factor III (thromboplastin tissue factor) antibody
see all
Images
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Tissue Factor was immunoprecipitated from 0.35 mg of mouse brain lysate with ab189483 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab189483 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse brain lysate 10 µg (Input).
Lane 2: ab189483 IP in mouse brain lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab1189483 in mouse brain lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189483).
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All lanes : Anti-Tissue Factor antibody [EPR18160-175] (ab189483) at 1/1000 dilution
Lane 1 : Mouse skin tissue lysate
Lane 2 : Mouse brain tissue lysate
Lanes 3-4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33, 47 kDa why is the actual band size different from the predicted?Blocking/Dilution: 5% NFDM/TBST.
Exposure: Lanes 1 and 3: 3 minutes; Lanes 2 and 4: 10 seconds.
The observed molecular mass is consistent with the literature (PMID: 7992837).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189483).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab227910 has not yet been referenced specifically in any publications.