Overview

  • Product name
    Anti-Tissue Factor antibody [EPR20369]
    See all Tissue Factor primary antibodies
  • Description
    Rabbit monoclonal [EPR20369] to Tissue Factor
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Tissue Factor aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P13726

  • Positive control
    • WB: Human Tissue Factor His-tagged recombinant protein (aa34-251); MDA-MB-231 and U-87 MG whole cell lysates; Human fetal kidney and lung cancer lysates. IHC-P: Human placenta. kidney and pancreas cancer tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab211016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function
    Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
  • Sequence similarities
    Belongs to the tissue factor family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD142 antibody
    • CD142 antigen antibody
    • Coagulation factor III (thromboplastin tissue factor) antibody
    • Coagulation factor III antibody
    • F3 antibody
    • FLJ17960 antibody
    • TF antibody
    • TF_HUMAN antibody
    • TFA antibody
    • Thromboplastin antibody
    • Tissue factor antibody
    see all

Images

  • All lanes : Anti-Tissue Factor antibody [EPR20369] (ab211016) at 1/1000 dilution (Anti-Tissue Factor antibody NPD)

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : Tissue Factor knockout HAP1 whole cell lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 33 kDa
    Observed band size: 39 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 2: Merged signal (red and green). Green - ab211016 observed at 49 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab211016 was shown to recognize Tissue Factor in wild-type HAP1 cells as signal was lost at the expected MW in Tissue Factor knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and F3 (Tissue Factor) knockout samples were subjected to SDS-PAGE. Ab211016 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Tissue Factor with ab211016 at 1/50 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

    Cytoplasmic staining on human kidney glomerulus is observed [PMID: 2237820].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Tissue Factor with ab211016 at 1/50 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

    Cytoplasmic staining on human placenta is observed [PMID: 2237820].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

  • Anti-Tissue Factor antibody [EPR20369] (ab211016) at 1/1000 dilution + Human Tissue Factor His-tagged recombinant protein (aa34-251) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-Tissue Factor antibody [EPR20369] (ab211016) at 1/5000 dilution

    Lane 1 : MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    MCF7 is a negative control (PMID: 24137414).

  • Lanes 1-2 : Anti-Tissue Factor antibody [EPR20369] (ab211016) at 1/1000 dilution
    Lane 3 : Anti-Tissue Factor antibody [EPR20369] (ab211016) at 1/10000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human lung cancer lysate
    Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The observed molecular weight is consistent with the literature (PMID: 7992837).

  • Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue labeling Tissue Factor with ab211016 at 1/50 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

    Membranous and cytoplasmic staining on tumor cells of human pancreas cancer is observed [PMID: 7648165].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

References

ab211016 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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