Key features and details
- Mouse monoclonal [MM0700-21U4] to Tissue Factor
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
- Isotype: IgG2
Product nameAnti-Tissue Factor antibody [MM0700-21U4]
See all Tissue Factor primary antibodies
DescriptionMouse monoclonal [MM0700-21U4] to Tissue Factor
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Tissue Factor aa 34-250.
GTTNTVAAYNLTWKSTNFKTILEWEPKPVNQVYTVQISTKSGDWKSKCFY TTDTECDLTDEIVKDVKQTYLARVFSYPAGNVESTGSAGEPLYENSPEFT PYLETNLGQPTIQSFEQVGTKVNVTVEDERTLVRRNNTFLSLRDVFGKDL IYTLYYWKSSSSGKKTAKTNTNEFLIDVDKGENYCFSVQAVIPSRTVNRK STDSPVECMGQEKGEFR
Database link: P13726
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: 100% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab201598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 33 kDa.|
|IP||Use at an assay dependent concentration.|
FunctionInitiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Sequence similaritiesBelongs to the tissue factor family.
- Information by UniProt
- CD142 antibody
- CD142 antigen antibody
- Coagulation factor III (thromboplastin tissue factor) antibody
Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
Lane 2: Tissue Factor knockout HAP1 whole cell lysate (40 µg)
Lanes 1 - 2: Merged signal (red and green). Green - ab201598 observed at 49 kDa. Red - loading control, ab176560, observed at 50 kDa.
ab201598 was shown to recognize Tissue Factor in wild-type HAP1 cells as signal was lost at the expected MW in Tissue Factor knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Tissue Factor knockout samples were subjected to SDS-PAGE. Ab201598 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
ab201598 has not yet been referenced specifically in any publications.