Tissue Factor Human Chromogenic Activity Assay Kit (ab108906)


  • Product name
    Tissue Factor Human Chromogenic Activity Assay Kit
    See all Tissue Factor kits
  • Sample type
    Urine, Serum, Plasma, Cell culture extracts, Tissue
  • Assay type
  • Species reactivity
    Reacts with: Human
  • Product overview

    The transmembrane protein Tissue factor (TF) is the physiologic trigger of coagulation in normal hemostasis. Tissue factor binds and allosterically activates factor VII (FVII). The TF-FVIIa complex cleaves factor IX and X, leading to thrombin generation. Tissue factor markedly enhances the ability of FVIIa to cleave both macromolecule and small peptidyl substrates. Inducible expression of Tissue factor in a variety of pathological conditions, including gram-negative sepsis and acute coronary syndromes, is associated with life-threatening thrombosis. In sepsis, Tissue factor expression within the vasculature leads to disseminated intravascular coagulation. Tissue factor also plays important roles in vasculogenesis, metastasis, and tumor-associated angiogenesis.


    ab108906 Tissue Factor Human Chromogenic Activity Assay kit is developed to determine Human Tissue Factor activity in plasma, tissue and cell culture supernatants. The assay measures the ability of lipoprotein TF/FVIIa to activate factor X (FX) to factor Xa. The amidolytic activity of the TF/FVIIa complex is quantitated by the amount of FXa produced using a highly specific FXa substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA at 405 nm is directly proportional to the TF enzymatic activity.



  • Colorimetric standard curve: mean of duplicates (+/- SD)

  • Standard curve showing concentration (pM) on X-axis and increase of O.D. per min on Y-axis (time frame: 20 min.)

  • Time course showing O.D.'s of one standard point and one sample (undiluted vs.diluted)

  • Tissue Factor measured in various biologicals showing average concentrations



ab108906 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

The standard should be reconstituted in 3ml water.

After arrival please store the individual components as instructed in the protocol booklet:

ab108906 may be stored until the expiration date at 2 to 8°C from the date of shipment. Store Standard, Factor VII protein, Factor X pro...

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Since tissue factor is both secreted and membrane bound, we recommend using cell lysates so the protein is properly released from the membrane.

As mentioned in the protocol under step 6.2) Cells lysates can be prepared as follows:

1. ...

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The labs says that the kit contains a low binding plate which will be better. However, otherwise the brand of the 96-well plate does not matter.

Thank you for contacting us on Friday in regards to the Tissue Factor Human Chromogenic Activity Assay Kit (ab108906).

I can now inform you that the vial of Tissue Factor Standard you received required the addition of 1.2 ml of reagent grade...

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The amount of standard is only provided on the vial label. The datasheet and the protocol bookletare only generic.
Please ask your customer tocheck the vial label, from which they can work out the volume of buffer they need to to generate a s...

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Thank you very much for contacting us.
I just wanted to let you know that I am still working on your inquiry. I am looking forthe information you requested.
I will forward to you the information as soon as I receive it. Thank you for your pat...

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I have just been informed that the tissue factor standard should be reconstituted with 1.2 ml of reagent grade water to generate a solution of 250 pM.


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