Key features and details
- Rabbit polyclonal to Tissue kallikrein
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-Tissue kallikrein antibody
See all Tissue kallikrein primary antibodies
DescriptionRabbit polyclonal to Tissue kallikrein
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse
Recombinant full length protein corresponding to Mouse Tissue kallikrein aa 25-261.
IVGGFNCEKNSQPWQVAVYRFTKYQCGGILLNANWVLTAAHCHNDKYQVW LGKNNFLEDEPSAQHRLVSKAIPHPDFNMSLLNEHTPQPEDDYSNDLMLL RLKKPADITDVVKPIDLPTEEPKLGSTCLASGWGSITPVKYEYPDELQCV NLKLLPNEDCAKAHIEKVTDDMLCAGDMDGGKDTCAGDSGGPLICDGVLQ GITSWGPSPCGKPNVPGIYTRVLNFNTWIRETMAEND
Database link: P15947
- Mouse kidney tissue.
Protein previously labeled as Kallikrein 1.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.025% Sodium azide
Constituents: 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab191014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 29 kDa.
The detection limit for Tissue kallikrein is approximately 0.5ng/lane under reducing conditions.
FunctionGlandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Tissue specificityIsoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84.
Sequence similaritiesBelongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain.
modificationsThe O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In PubMed:3163150, GalNAc was detected with the corresponding peptides but not located.
- Information by UniProt
- Glandular kallikrein 1 antibody
- hK 1 antibody
- hK1 antibody
ab191014 has not yet been referenced specifically in any publications.