• Product name

    Anti-Tissue kallikrein antibody
    See all Tissue kallikrein primary antibodies
  • Description

    Rabbit polyclonal to Tissue kallikrein
  • Host species

  • Specificity

    ab28289 recognises the Kallikrein loop of Tissue kallikrein. It does not react with the other human Kallikreins.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Tissue kallikrein. Corresponding to the the Kallikrein loop area of Tissue kallikrein.
    (Peptide available as ab41419)

  • General notes

    Protein previously labeled as Kallikrein 1.



Our Abpromise guarantee covers the use of ab28289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 28 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.


  • Function

    Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
  • Tissue specificity

    Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84.
  • Sequence similarities

    Belongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • Post-translational

    The O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In PubMed:3163150, GalNAc was detected with the corresponding peptides but not located.
  • Information by UniProt
  • Database links

  • Alternative names

    • Glandular kallikrein 1 antibody
    • hK 1 antibody
    • hK1 antibody
    • Kallikrein serine protease 1 antibody
    • Kallikrein-1 antibody
    • Kidney/pancreas/salivary gland kallikrein antibody
    • Klk 6 antibody
    • KLK1 antibody
    • KLK1_HUMAN antibody
    • Klk6 antibody
    • KLKR antibody
    • Tissue kallikrein antibody
    see all


  • All lanes : Anti-Tissue kallikrein antibody (ab28289) at 1/1000 dilution

    Lane 1 : Kalikrein 1 at 0.05 µg
    Lane 2 : Kalikrein 1 at 0.01 µg
    Lane 3 : Kalikrein 1 at 0.001 µg

    Predicted band size: 28 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?

  • IHC image of ab28289 staining in human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab28289, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:

  • Tang SC  et al. Bradykinin and high glucose promote renal tubular inflammation. Nephrol Dial Transplant 25:698-710 (2010). ICC/IF ; Human . Read more (PubMed: 19923143) »
  • Cassim B  et al. Kallikreins, kininogens and kinin receptors on circulating and synovial fluid neutrophils: role in kinin generation in rheumatoid arthritis. Rheumatology (Oxford) 48:490-6 (2009). ICC/IF, IHC ; Human . Read more (PubMed: 19254919) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Following up my previous message, immunogens for the kallikrein antibodies ab28289 and ab40497 were aligned with the mouse canonical sequence (http://www.uniprot.org/uniprot/P15947.fasta). The ab28289 epitope falls in a region of relatively low sequence homology, approximately 48 % identical, so the antibody is unlikely to recognize the mouse kallikrein very well, if at all. The ab40947 immunogen homology is better, with 66% identity, and may recognize the mouse protein, although not as well as the human protein. I hope this is helpful. If you have any questions, please contact us.

Read More


Thank you for contacting us. I am still waiting for our source for the kallikrein antibodies ab28289 and ab40497 to send their alignments of the immunogens and mouse kallikrein but I have checked the homology of the immunogens for the other four antibodies against the corresponding mouse sequences. For the third kallikrein antibody we discussed, ab59520, the homology is relatively low, with only 58% identity: PN++C KAH++K The letters are the amino acids that are conserved and the "+" symbols are biochemically similar amino acids, but not identical. There is some chance of cross-reaction but we feel it is unlikely. For the remaining three antibodies, ab103013 anti-PTPRD, ab1100844 anti-troponin C, and ab116610 anti-ITIH4, the alignment results are as follows: ab103013: 93% identity ab110844: 99% identity ab116610: 50% identity. The first two are likely to react with the mouse proteins, but we do not guarantee this. I hope this information is helpful to you. I will send the alignment results fro the other two KLK1 antibodies when I receive them. Please do not hesitate to contact us if you need any more advice or information.

Read More

For licensing inquiries, please contact partnerships@abcam.com

Sign up