Anti-Tissue kallikrein antibody (ab28289)
Key features and details
- Rabbit polyclonal to Tissue kallikrein
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Tissue kallikrein antibody
See all Tissue kallikrein primary antibodies -
Description
Rabbit polyclonal to Tissue kallikrein -
Host species
Rabbit -
Specificity
ab28289 recognises the Kallikrein loop of Tissue kallikrein. It does not react with the other human Kallikreins. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Tissue kallikrein. Corresponding to the the Kallikrein loop area of Tissue kallikrein.
(Peptide available asab41419) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 28 kDa.
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 28 kDa. |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. -
Tissue specificity
Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84. -
Sequence similarities
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsThe O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In PubMed:3163150, GalNAc was detected with the corresponding peptides but not located. - Information by UniProt
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Database links
- Entrez Gene: 3816 Human
- Omim: 147910 Human
- SwissProt: P06870 Human
- Unigene: 123107 Human
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Alternative names
- Glandular kallikrein 1 antibody
- hK 1 antibody
- hK1 antibody
see all
Images
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All lanes : Anti-Tissue kallikrein antibody (ab28289) at 1/1000 dilution
Lane 3 : Kallikrein-1 (rhK-1) at 0.05 µg
Lane 4 : Kallikrein-1 (rhK-1) at 0.01 µg
Lane 5 : Kallikrein-1 (rhK-1) at 0.001 µg
Lane 7 : Kallikrein-3 (rhK-3) at 0.05 µg
Lane 8 : Kallikrein-3 (rhK-3) at 0.01 µg
Lane 9 : Kallikrein-3 (rhK-3) at 0.001 µg
Lane 11 : Kallikrein-4 (rhK-4) at 0.05 µg
Lane 12 : Kallikrein-4 (rhK-4) at 0.01 µg
Lane 13 : Kallikrein-4 (rhK-4) at 0.001 µg
Predicted band size: 28 kDa -
IHC image of ab28289 staining in human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab28289, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab28289 has been referenced in 4 publications.
- Huang S et al. Co-expression of tissue kallikrein 1 and tissue inhibitor of matrix metalloproteinase 1 improves myocardial ischemia-reperfusion injury by promoting angiogenesis and inhibiting oxidative stress. Mol Med Rep 23:N/A (2021). PubMed: 33355364
- Zhang M et al. Human Tissue Kallikrein 1 Is Downregulated in Elderly Human Prostates and Possesses Potential In Vitro Antioxidative and Antifibrotic Effects in Rodent Prostates. Oxid Med Cell Longev 2021:8877540 (2021). PubMed: 34007408
- Tang SC et al. Bradykinin and high glucose promote renal tubular inflammation. Nephrol Dial Transplant 25:698-710 (2010). ICC/IF ; Human . PubMed: 19923143
- Cassim B et al. Kallikreins, kininogens and kinin receptors on circulating and synovial fluid neutrophils: role in kinin generation in rheumatoid arthritis. Rheumatology (Oxford) 48:490-6 (2009). ICC/IF, IHC ; Human . PubMed: 19254919