Anti-Tissue Plasminogen Activator antibody (ab28219)

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Key features and details

  • Rabbit polyclonal to Tissue Plasminogen Activator
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Tissue Plasminogen Activator antibody
    See all Tissue Plasminogen Activator primary antibodies

  • Description

    Rabbit polyclonal to Tissue Plasminogen Activator

  • Host species

    Rabbit

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length TPA Tissue Plasminogen Activator protein (Human).

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Target

  • Function

    Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration.

  • Tissue specificity

    Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.

  • Involvement in disease

    Note=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism.

  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 fibronectin type-I domain.
    Contains 2 kringle domains.
    Contains 1 peptidase S1 domain.

  • Domain

    Both FN1 and one of the kringle domains are required for binding to fibrin.
    Both FN1 and EGF-like domains are important for binding to LRP1.
    The FN1 domain mediates binding to annexin A2.
    The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site.

  • Post-translational
    modifications

    The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
    Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
    N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
    Characterization of O-linked glycan was studied in Bowes melanoma cell line.

  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt

  • Database links

    • Alternative names

      • Alteplase antibody
      • DKFZp686I03148 antibody
      • Plasminogen activator tissue antibody
      • Plasminogen activator tissue type antibody
      • PLAT antibody
      • Reteplase antibody
      • t PA antibody
      • T Plasminogen Activator antibody
      • t-PA antibody
      • T-plasminogen activator antibody
      • Tissue plasminogen activator (t PA) antibody
      • Tissue type plasminogen activator antibody
      • Tissue-type plasminogen activator chain B antibody
      • tPA antibody
      • TPA_HUMAN antibody
      • TPA1 antibody
      see all

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (2)

    Publishing research using ab28219? Please let us know so that we can cite the reference in this datasheet.

    ab28219 has been referenced in 2 publications.

    • Maynadier M  et al. Cathepsin D stimulates the activities of secreted plasminogen activators in the breast cancer acidic environment. Int J Oncol 43:1683-90 (2013). PubMed: 24026424
    • Chenau J  et al. The cell line secretome, a suitable tool for investigating proteins released in vivo by tumors: application to the study of p53-modulated proteins secreted in lung cancer cells. J Proteome Res 8:4579-91 (2009). WB ; Human . PubMed: 19639960

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Question

    Please let me know the concentration of the above antibody.

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    Answer

    Thank you for contacting us.

    This antibody has not been purified and therefore, its concentration has not been determined. However we estimate that its concentration will be in the region of 1-10 mg/ml.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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    Question

    Thank you very much for your answer. I hope they are still ok as well. We do see indeed a bit more background than 2 weeks ago, but it might also be due to the sample. We blot with iBlot (invitrogen), block the mamabrane for 4 h and incubate with primary antibody overnight in the fridge without shaking. Further western blot is performed wth WesternBreeze kit from invitrogen, so after incubation with each anitbody there are 3 short washes. We could do additional washing step between applying antibodies. If you have any tips, you are welcome.  

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    Answer

    I do have a few recommendations that might help. One thing you can do is block the membranes overnight at four degrees Celsius which can help reduce background. Also, incubating the primary antibody with blocking solution that also has 0.01% tween 20 can also help reduce non-specific binding. Adding tween to your washing fluid is also effective. Lastly, I would recommend that you use a semi dry transfer method or a full immersion transfer. We have heard from some customers that using the iBlot system can also contribute to background staining on the blot. I hope that you find this information helpful. Please feel free to contact us again should you have further questions about this antibody or any of the products in our catalogue.    

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    Question

    I'm writing with question regarding storage of primary antibodies AB 28219. Around 5 weeks ago we boght 7 vials from you. Package was labelled with storage conditions of 2-8 degrees and this is how they were stored for 5 weeks. Now we have found the information that antibodies should be aliquoted and stored at -80 degrees. Can you speculate if storage at 2-8 degress for 5 weeks has seviere implications on the stability/sensitivity and performance of this antibodies ?  

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    Answer

    Thank you for contacting Abcam. I am sorry for the confusion about the storage conditions. I think the antibodies will most likely be fine if you can aliquot them and get them in a freezer. We have done several tests with the stability of our antibodies and know it to be quite good. The recommended storage on the datasheet is important but more so for long term storage. Having said that you may notice a bit more background or non-specific staining with these antibodies, but that issue can usual be solved by adjusting your blocking, washing or primary incubation methods.    

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