Anti-Tissue Plasminogen Activator antibody (ab62763)

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Western blot - Anti-Tissue Plasminogen Activator antibody (ab62763)
  • Western blot - Anti-Tissue Plasminogen Activator antibody (ab62763)

Key features and details

  • Rabbit polyclonal to Tissue Plasminogen Activator
  • Suitable for: WB
  • Reacts with: Mouse, Rat
  • Isotype: IgG

Overview

  • Product name

    Anti-Tissue Plasminogen Activator antibody
    See all Tissue Plasminogen Activator primary antibodies

  • Description

    Rabbit polyclonal to Tissue Plasminogen Activator

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant full length protein corresponding to Mouse TPA Tissue Plasminogen Activator.

  • Positive control

    • WB: Mouse TPA Tissue Plasminogen Activator (non-reduced).

  • General notes

    The concentration of this antibody is lot specific.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

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Properties

Applications

Our Abpromise guarantee covers the use of ab62763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 62 kDa).

Target

  • Function

    Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration.

  • Tissue specificity

    Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.

  • Involvement in disease

    Note=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism.

  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 fibronectin type-I domain.
    Contains 2 kringle domains.
    Contains 1 peptidase S1 domain.

  • Domain

    Both FN1 and one of the kringle domains are required for binding to fibrin.
    Both FN1 and EGF-like domains are important for binding to LRP1.
    The FN1 domain mediates binding to annexin A2.
    The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site.

  • Post-translational
    modifications

    The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
    Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
    N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
    Characterization of O-linked glycan was studied in Bowes melanoma cell line.

  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt

  • Database links

    • Alternative names

      • Alteplase antibody
      • DKFZp686I03148 antibody
      • Plasminogen activator tissue antibody
      • Plasminogen activator tissue type antibody
      • PLAT antibody
      • Reteplase antibody
      • t PA antibody
      • T Plasminogen Activator antibody
      • t-PA antibody
      • T-plasminogen activator antibody
      • Tissue plasminogen activator (t PA) antibody
      • Tissue type plasminogen activator antibody
      • Tissue-type plasminogen activator chain B antibody
      • tPA antibody
      • TPA_HUMAN antibody
      • TPA1 antibody
      see all

    Images

    • Western blot - Anti-Tissue Plasminogen Activator antibody (ab62763)
      Western blot - Anti-Tissue Plasminogen Activator antibody (ab62763)
      All lanes : Anti-Tissue Plasminogen Activator antibody (ab62763) at 2 µg/ml

      Lane 1 : Mouse TPA Tissue Plasminogen Activator (non-reduced) at 0.5 µg
      Lane 2 : Mouse TPA Tissue Plasminogen Activator + 3 µg CPAI (non-reduced) at 0.5 µg
      Lane 3 : Mouse TPA Tissue Plasminogen Activator (reduced) at 0.5 µg
      Lane 4 : Mouse TPA Tissue Plasminogen Activator + 3ug CPAI (reduced) at 0.5 µg
      Lane 5 : Marker

      Secondary
      All lanes : Anti Rabbit IgG ABC Kit

      Predicted band size: 62 kDa
      Observed band size: 75 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 100 kDa, 125 kDa, 38 kDa, 85 kDa. We are unsure as to the identity of these extra bands.

    • Western blot - Anti-Tissue Plasminogen Activator antibody (ab62763)
      Western blot - Anti-Tissue Plasminogen Activator antibody (ab62763)

    References (2)

    Publishing research using ab62763? Please let us know so that we can cite the reference in this datasheet.

    ab62763 has been referenced in 2 publications.

    • Lenoir S  et al. Post-synaptic Release of the Neuronal Tissue-Type Plasminogen Activator (tPA). Front Cell Neurosci 13:164 (2019). PubMed: 31105531
    • Sagare AP  et al. A lipoprotein receptor cluster IV mutant preferentially binds amyloid-ß and regulates its clearance from the mouse brain. J Biol Chem 288:15154-66 (2013). PubMed: 23580652

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