RecombinantRabMAb

Recombinant Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)
  • Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7232(2)] to Tissue Plasminogen Activator - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free
    See all Tissue Plasminogen Activator primary antibodies

  • Description

    Rabbit monoclonal [EPR7232(2)] to Tissue Plasminogen Activator - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human TPA Tissue Plasminogen Activator. The exact sequence is proprietary.

  • Positive control

    • IHC-P: human lung carcinoma, cervical carcinoma, skeletal muscle, brain, kidney and liver tissue, mouse and rat liver tissue

  • General notes

    Ab240146 is the carrier-free version of ab157469. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240146 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab240146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 63 kDa.
IHC-P Use at an assay dependent concentration.

Perform heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration.

    • Tissue specificity

      Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.

    • Involvement in disease

      Note=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism.

    • Sequence similarities

      Belongs to the peptidase S1 family.
      Contains 1 EGF-like domain.
      Contains 1 fibronectin type-I domain.
      Contains 2 kringle domains.
      Contains 1 peptidase S1 domain.

    • Domain

      Both FN1 and one of the kringle domains are required for binding to fibrin.
      Both FN1 and EGF-like domains are important for binding to LRP1.
      The FN1 domain mediates binding to annexin A2.
      The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site.

    • Post-translational
      modifications

      The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
      Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
      N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
      Characterization of O-linked glycan was studied in Bowes melanoma cell line.

    • Cellular localization

      Secreted > extracellular space.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Alteplase antibody
      • DKFZp686I03148 antibody
      • Plasminogen activator tissue antibody
      • Plasminogen activator tissue type antibody
      • PLAT antibody
      • Reteplase antibody
      • t PA antibody
      • T Plasminogen Activator antibody
      • t-PA antibody
      • T-plasminogen activator antibody
      • Tissue plasminogen activator (t PA) antibody
      • Tissue type plasminogen activator antibody
      • Tissue-type plasminogen activator chain B antibody
      • tPA antibody
      • TPA_HUMAN antibody
      • TPA1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling TPA Tissue Plasminogen Activator with purified ab157469 at 1/1000 dilution (1.91 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157469)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling TPA Tissue Plasminogen Activator with purified ab157469 at 1/1000 dilution (1.91 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157469)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling TPA Tissue Plasminogen Activator with purified ab157469 at 1/1000 dilution (1.91 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157469)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)

      ab157469 (unpurified) showing +ve staining in Human normal liver.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157469).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)

      ab157469 (unpurified) showing +ve staining in Human normal kidney.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157469).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)

      ab157469 (unpurified) showing +ve staining in Human normal brain.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157469).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)

      ab157469 (unpurified) showing +ve staining in Human skeletal muscle.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157469).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)

      Immunohistochemical analysis of paraffin-embedded Human cervial carcinoma tissue labeling TPA Tissue Plasminogen Activator with ab157469 (unpurified) 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157469).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TPA Tissue Plasminogen Activator with ab157469 (unpurified) at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157469).

    • Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)
      Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (ab240146)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab240146? Please let us know so that we can cite the reference in this datasheet.

    ab240146 has not yet been referenced specifically in any publications.

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