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    titin-antibody-ab203623.pdf

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Epigenetics and Nuclear Signaling Chromosome Structure Chromosome
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Anti-Titin antibody (ab203623)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Titin antibody (ab203623)

    Key features and details

    • Rabbit polyclonal to Titin
    • Suitable for: IHC-P
    • Reacts with: Rat
    • Isotype: IgG

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    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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    Overview

    • Product name

      Anti-Titin antibody
      See all Titin primary antibodies
    • Description

      Rabbit polyclonal to Titin
    • Host species

      Rabbit
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Rat
      Predicted to work with: Mouse, Human
    • Immunogen

      Synthetic peptide within Human Titin aa 2670-2703 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary. (Ig-like 15 domain).
      Sequence:

      DGHKRRLIIAATKLDDIGEYTYKVATSKTSAKLK


      Database link: Q8WZ42
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • Rat heart tissue.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.09% Sodium azide
      Constituents: 50% Glycerol, 1% BSA
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Chromosome Structure
      • Chromosome
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Microfilaments
      • Actin etc
      • Actin Binding Proteins
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Motor Proteins
      • Myosin
      • Cardiovascular
      • Heart
      • Hypertrophy
      • Other
      • Cardiovascular
      • Heart
      • Contractility
      • Contractile Proteins
      • Actins
      • Cardiovascular
      • Heart
      • Contractility
      • Contractile Proteins
      • Myosins

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    Our Abpromise guarantee covers the use of ab203623 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Target

    • Function

      Key component in the assembly and functioning of vertebrate striated muscles. By providing connections at the level of individual microfilaments, it contributes to the fine balance of forces between the two halves of the sarcomere. The size and extensibility of the cross-links are the main determinants of sarcomere extensibility properties of muscle. In non-muscle cells, seems to play a role in chromosome condensation and chromosome segregation during mitosis. Might link the lamina network to chromatin or nuclear actin, or both during interphase.
    • Tissue specificity

      Isoform 3, isoform 7 and isoform 8 are expressed in cardiac muscle. Isoform 4 is expressed in vertebrate skeletal muscle. Isoform 6 is expressed in cardiac tissues.
    • Involvement in disease

      Defects in TTN are the cause of hereditary myopathy with early respiratory failure (HMERF) [MIM:603689]; also known as Edstrom myopathy. HMERF is an autosomal dominant, adult-onset myopathy with early respiratory muscle involvement.
      Defects in TTN are the cause of familial hypertrophic cardiomyopathy type 9 (CMH9) [MIM:613765]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
      Defects in TTN are the cause of cardiomyopathy dilated type 1G (CMD1G) [MIM:604145]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
      Defects in TTN are the cause of tardive tibial muscular dystrophy (TMD) [MIM:600334]; also known as Udd myopathy. TMD is an autosomal dominant, late-onset distal myopathy. Muscle weakness and atrophy are usually confined to the anterior compartment of the lower leg, in particular the tibialis anterior muscle. Clinical symptoms usually occur at age 35-45 years or much later.
      Defects in TTN are the cause of limb-girdle muscular dystrophy type 2J (LGMD2J) [MIM:608807]. LGMD2J is an autosomal recessive degenerative myopathy characterized by progressive weakness of the pelvic and shoulder girdle muscles. Severe disability is observed within 20 years of onset.
      Defects in TTN are the cause of early-onset myopathy with fatal cardiomyopathy (EOMFC) [MIM:611705]. Early-onset myopathies are inherited muscle disorders that manifest typically from birth or infancy with hypotonia, muscle weakness, and delayed motor development. EOMFC is a titinopathy that, in contrast with the previously described examples, involves both heart and skeletal muscle, has a congenital onset, and is purely recessive. This phenotype is due to homozygous out-of-frame TTN deletions, which lead to a total absence of titin's C-terminal end from striated muscles and to secondary CAPN3 depletion.
    • Sequence similarities

      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
      Contains 132 fibronectin type-III domains.
      Contains 152 Ig-like (immunoglobulin-like) domains.
      Contains 19 Kelch repeats.
      Contains 1 protein kinase domain.
      Contains 17 RCC1 repeats.
      Contains 14 TPR repeats.
      Contains 15 WD repeats.
    • Domain

      ZIS1 and ZIS5 regions contain multiple SPXR consensus sites for ERK- and CDK-like protein kinases as well as multiple SP motifs. ZIS1 could adopt a closed conformation which would block the TCAP-binding site.
      The PEVK region may serve as an entropic spring of a chain of structural folds and may also be an interaction site to other myofilament proteins to form interfilament connectivity in the sarcomere.
    • Post-translational
      modifications

      Autophosphorylated (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Target information above from: UniProt accession Q8WZ42 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7273 Human
      • Entrez Gene: 22138 Mouse
      • Entrez Gene: 84015 Rat
      • Omim: 188840 Human
      • SwissProt: Q8WZ42 Human
      • SwissProt: A2ASS6 Mouse
      • Unigene: 134602 Human
      • Unigene: 373672 Mouse
      • Unigene: 446279 Mouse
      • Unigene: 473358 Mouse
      • Unigene: 232176 Rat
      • Unigene: 232712 Rat
      • Unigene: 232713 Rat
      • Unigene: 232714 Rat
      • Unigene: 232729 Rat
      • Unigene: 4008 Rat
      see all
    • Alternative names

      • MPRM antibody
      • Cardiomyopathy dilated 1G (autosomal dominant) antibody
      • CMD1G antibody
      • CMH 9 antibody
      • CMH9 antibody
      • CMPD 4 antibody
      • CMPD4 antibody
      • Connectin antibody
      • DKFZp451N061 antibody
      • EOMFC antibody
      • FLJ26020 antibody
      • FLJ26409 antibody
      • FLJ32040 antibody
      • FLJ34413 antibody
      • FLJ39564 antibody
      • FLJ43066 antibody
      • HMERF antibody
      • LGMD2J antibody
      • MU RMS 40.14 antibody
      • MYLK5 antibody
      • Rhabdomyosarcoma antigen antibody
      • Rhabdomyosarcoma antigen MU RMS 40.14 antibody
      • Rhabdomyosarcoma antigen MU-RMS-40.14 antibody
      • Titin antibody
      • TITIN_HUMAN antibody
      • TMD antibody
      • TTN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Titin antibody (ab203623)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Titin antibody (ab203623)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat heart tissue labeling Titin with ab203623 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab203623? Please let us know so that we can cite the reference in this datasheet.

    ab203623 has not yet been referenced specifically in any publications.

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