Key features and details
- Rabbit polyclonal to TJP2/ZO2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TJP2/ZO2 antibody
See all TJP2/ZO2 primary antibodies
DescriptionRabbit polyclonal to TJP2/ZO2
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Dog
- WB: A431 cell lysate. ICC/IF: U-2 OS cells. IHC-P: Human colon tissue.
This product was previously labelled as TJP2
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 134 kDa.|
FunctionPlays a role in tight junctions and adherens junctions.
Tissue specificityThis protein is found in epithelial cell junctions. Isoform A1 is abundant in the heart and brain. Detected in brain and skeletal muscle. It is present almost exclusively in normal tissues. Isoform C1 is expressed at high level in the kidney, pancreas, heart and placenta. Not detected in brain and skeletal muscle. Found in normal as well as in most neoplastic tissues.
Involvement in diseaseDefects in TJP2 are involved in familial hypercholanemia (FHCA) [MIM:607748]. FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption.
Sequence similaritiesBelongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain.
Cellular localizationCell junction > adherens junction. Cell membrane. Cell junction > tight junction. Nucleus. Also nuclear under environmental stress conditions and in migratory endothelial cells and subconfluent epithelial cell cultures.
- Information by UniProt
- C9DUPq21.11 antibody
- DFNA51 antibody
- DUP9q21.11 antibody
All lanes : ab224314 at 0.4 µg/ml
Lane 1 : Human A-431 cell lines
Lane 2 : Human SK-MEL-30 cell line
Predicted band size: 134 kDa
Loading control: Anti-HSP90B1
Paraffin-embedded human testis stained for TJP2 using ab224314 at 1/50 dilution in immunohistochemical analysis. Shows strong cytoplasmic and membranous positivity in cells in seminiferous ducts.
PFA-fixed/Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for TJP2/ZO2 (green) using ab224314 (4 µg/ml) in ICC/IF.
Paraffin-embedded human pancreas stained for TJP2 using ab224314 at 1/50 dilution in immunohistochemical analysis. Shows weak cytoplasmic positivity in exocrine glandular cells.
Paraffin-embedded human cerebral cortex stained for TJP2 using ab224314 at 1/50 dilution in immunohistochemical analysis. Shows strong cytoplasmic positivity in glial cells.
Paraffin-embedded human colon stained for TJP2 using ab224314 at 1/50 dilution in immunohistochemical analysis. Shows moderate cytoplasmic and membranous positivity in glandular cells.
ab224314 has not yet been referenced specifically in any publications.