Key features and details
- Rabbit polyclonal to TJP2/ZO2 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-TJP2/ZO2 antibody - C-terminal
See all TJP2/ZO2 primary antibodies
DescriptionRabbit polyclonal to TJP2/ZO2 - C-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat
- Mouse liver tissue; Rat brain, Mouse brain tissue lysates; PC12 and HEPA cell lysates.
This product was previously labelled as TJP2
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab191133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 131 kDa.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPlays a role in tight junctions and adherens junctions.
Tissue specificityThis protein is found in epithelial cell junctions. Isoform A1 is abundant in the heart and brain. Detected in brain and skeletal muscle. It is present almost exclusively in normal tissues. Isoform C1 is expressed at high level in the kidney, pancreas, heart and placenta. Not detected in brain and skeletal muscle. Found in normal as well as in most neoplastic tissues.
Involvement in diseaseDefects in TJP2 are involved in familial hypercholanemia (FHCA) [MIM:607748]. FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption.
Sequence similaritiesBelongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain.
Cellular localizationCell junction > adherens junction. Cell membrane. Cell junction > tight junction. Nucleus. Also nuclear under environmental stress conditions and in migratory endothelial cells and subconfluent epithelial cell cultures.
- Information by UniProt
- C9DUPq21.11 antibody
- DFNA51 antibody
- DUP9q21.11 antibody
All lanes : Anti-TJP2/ZO2 antibody - C-terminal (ab191133) at 0.5 µg/ml
Lane 1 : Rat brain tissue lysate
Lane 2 : PC12 cell lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : HEPA cell lysate
Predicted band size: 131 kDa
The detection limit for TJP2/ZO2 is approximately 2.5 ng/lane under reducing conditions.
Immunohistochemical analysis of paraffin embedded Mouse liver tissue labeling TJP2/ZO2 using ab191133 at 1 μg/ml.
ab191133 has been referenced in 1 publication.
- Peng H et al. Qihuang decoction promotes the recovery of intestinal immune barrier dysfunction after gastrectomy in rats. Am J Transl Res 10:827-836 (2018). WB ; Rat . PubMed: 29636872