Overview

  • Product name

    Anti-TJP2/ZO2 antibody - C-terminal
    See all TJP2/ZO2 primary antibodies
  • Description

    Rabbit polyclonal to TJP2/ZO2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse TJP2/ZO2 aa 1149-1167 (C terminal).
    Sequence:

    AHSKRGYYSQPSRYRDTEL


    Database link: Q9Z0U1

  • Positive control

    • Mouse liver tissue; Rat brain, Mouse brain tissue lysates; PC12 and HEPA cell lysates.
  • General notes

     This product was previously labelled as TJP2

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 131 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Plays a role in tight junctions and adherens junctions.
  • Tissue specificity

    This protein is found in epithelial cell junctions. Isoform A1 is abundant in the heart and brain. Detected in brain and skeletal muscle. It is present almost exclusively in normal tissues. Isoform C1 is expressed at high level in the kidney, pancreas, heart and placenta. Not detected in brain and skeletal muscle. Found in normal as well as in most neoplastic tissues.
  • Involvement in disease

    Defects in TJP2 are involved in familial hypercholanemia (FHCA) [MIM:607748]. FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption.
  • Sequence similarities

    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
  • Cellular localization

    Cell junction > adherens junction. Cell membrane. Cell junction > tight junction. Nucleus. Also nuclear under environmental stress conditions and in migratory endothelial cells and subconfluent epithelial cell cultures.
  • Information by UniProt
  • Database links

  • Alternative names

    • C9DUPq21.11 antibody
    • DFNA51 antibody
    • DUP9q21.11 antibody
    • Friedreich ataxia region gene X104 (tight junction protein ZO-2) antibody
    • MGC26306 antibody
    • PFIC4 antibody
    • Tight junction protein 2 antibody
    • Tight junction protein ZO 2 antibody
    • Tight junction protein ZO-2 antibody
    • TJP2 antibody
    • X104 antibody
    • ZO 2 antibody
    • ZO-2 antibody
    • ZO2 antibody
    • ZO2_HUMAN antibody
    • Zona occludens 2 antibody
    • Zona occludens protein 2 antibody
    • Zonula occludens protein 2 antibody
    see all

Images

  • All lanes : Anti-TJP2/ZO2 antibody - C-terminal (ab191133) at 0.5 µg/ml

    Lane 1 : Rat brain tissue lysate
    Lane 2 : PC12 cell lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : HEPA cell lysate

    Predicted band size: 131 kDa



    The detection limit for TJP2/ZO2 is approximately 2.5 ng/lane under reducing conditions.

  • Immunohistochemical analysis of paraffin embedded Mouse liver tissue labeling TJP2/ZO2 using ab191133 at 1 μg/ml.

References

This product has been referenced in:

  • Peng H  et al. Qihuang decoction promotes the recovery of intestinal immune barrier dysfunction after gastrectomy in rats. Am J Transl Res 10:827-836 (2018). WB ; Rat . Read more (PubMed: 29636872) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Human Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing
Loading amount
28 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 24 2015

Application
Western blot
Sample
Mouse Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount
28 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 24 2015

Application
Western blot
Sample
Rat Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing
Loading amount
28 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mr. Julien Chevalier

Verified customer

Submitted Jul 24 2015

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