Anti-TJP2/ZO2 antibody - C-terminal (ab191133)
Key features and details
- Rabbit polyclonal to TJP2/ZO2 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-TJP2/ZO2 antibody - C-terminal
See all TJP2/ZO2 primary antibodies -
Description
Rabbit polyclonal to TJP2/ZO2 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseWB MouseRat
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Immunogen
Synthetic peptide corresponding to Mouse TJP2/ZO2 aa 1100 to the C-terminus (C terminal).
Database link: Q9Z0U1 -
Positive control
- Mouse liver tissue; Rat brain, Mouse brain tissue lysates; PC12 and HEPA cell lysates.
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General notes
This product was previously labelled as TJP2
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Mouse
|
| WB |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 131 kDa.
|
| IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 131 kDa. |
|
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Plays a role in tight junctions and adherens junctions. -
Tissue specificity
This protein is found in epithelial cell junctions. Isoform A1 is abundant in the heart and brain. Detected in brain and skeletal muscle. It is present almost exclusively in normal tissues. Isoform C1 is expressed at high level in the kidney, pancreas, heart and placenta. Not detected in brain and skeletal muscle. Found in normal as well as in most neoplastic tissues. -
Involvement in disease
Defects in TJP2 are involved in familial hypercholanemia (FHCA) [MIM:607748]. FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption. -
Sequence similarities
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain. -
Cellular localization
Cell junction > adherens junction. Cell membrane. Cell junction > tight junction. Nucleus. Also nuclear under environmental stress conditions and in migratory endothelial cells and subconfluent epithelial cell cultures. - Information by UniProt
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Database links
- Entrez Gene: 21873 Mouse
- Entrez Gene: 115769 Rat
- SwissProt: Q9Z0U1 Mouse
- Unigene: 104744 Mouse
- Unigene: 10965 Rat
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Alternative names
- C9DUPq21.11 antibody
- DFNA51 antibody
- DUP9q21.11 antibody
see all
Images
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Western blot - Anti-TJP2/ZO2 antibody - C-terminal (ab191133)All lanes : Anti-TJP2/ZO2 antibody - C-terminal (ab191133) at 0.5 µg/ml
Lane 1 : Rat brain tissue lysate
Lane 2 : PC12 cell lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : HEPA cell lysate
Predicted band size: 131 kDaThe detection limit for TJP2/ZO2 is approximately 2.5 ng/lane under reducing conditions.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TJP2/ZO2 antibody - C-terminal (ab191133)Immunohistochemical analysis of paraffin embedded Mouse liver tissue labeling TJP2/ZO2 using ab191133 at 1 μg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab191133 has been referenced in 1 publication.
- Peng H et al. Qihuang decoction promotes the recovery of intestinal immune barrier dysfunction after gastrectomy in rats. Am J Transl Res 10:827-836 (2018). WB ; Rat . PubMed: 29636872