For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Rabbit polyclonal to TKTL1
Recombinant protein fragment, corresponding to a region within amino acids 1-311 of Human TKTL1 (UniProt ID: P51854).
- 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates; A431 cells; Human SAS xenograft tissue.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/1000 - 1/10000. Predicted molecular weight: 65 kDa.
||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
||1/100 - 1/1000.
Expressed in fetal and adult heart, brain, lung, liver, and kidney, and in adult placenta, skeletal muscle and pancreas. Up-regulated in various epithelial tumors.
Belongs to the transketolase family.
Cytoplasm. Nucleus. Predominantly cytoplasmic and to a lesser extent also nuclear.
Information by UniProt
- TK 2 antibody
- TK2 antibody
- TKR antibody
Western blot - Anti-TKTL1 antibody (ab155662)
All lanes : Anti-TKTL1 antibody (ab155662) at 1/5000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : MOLT4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 65 kDa
7.5% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TKTL1 antibody (ab155662)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human SAS xenograft tissue, labeling TKTL1 using ab155662 at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-TKTL1 antibody (ab155662)
Immunofluorescence analysis of paraformaldehyde-fixed A431 cells, labeling TKTL1 using ab155662 at a 1/200 dilution. The image in the lower panel was costained with Hoechst 33342.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"