Overview

  • Product name
    Anti-TKTL1 antibody
  • Description
    Rabbit polyclonal to TKTL1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protein fragment, corresponding to a region within amino acids 1-311 of Human TKTL1 (UniProt ID: P51854).

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates; A431 cells; Human SAS xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab155662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 65 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Tissue specificity
    Expressed in fetal and adult heart, brain, lung, liver, and kidney, and in adult placenta, skeletal muscle and pancreas. Up-regulated in various epithelial tumors.
  • Sequence similarities
    Belongs to the transketolase family.
  • Cellular localization
    Cytoplasm. Nucleus. Predominantly cytoplasmic and to a lesser extent also nuclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • TK 2 antibody
    • TK2 antibody
    • TKR antibody
    • TKT 2 antibody
    • TKT2 antibody
    • TKTL1 antibody
    • TKTL1_HUMAN antibody
    • Transketolase 2 antibody
    • Transketolase like 1 antibody
    • Transketolase like protein 1 antibody
    • Transketolase related protein antibody
    • transketolase-like 1 antibody
    • Transketolase-like protein 1 antibody
    • Transketolase-related protein antibody
    • Transketolase2 antibody
    see all

Images

  • All lanes : Anti-TKTL1 antibody (ab155662) at 1/5000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HepG2 whole cell lysate
    Lane 3 : MOLT4 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 65 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human SAS xenograft tissue, labeling TKTL1 using ab155662 at a 1/100 dilution.
  • Immunofluorescence analysis of paraformaldehyde-fixed A431 cells, labeling TKTL1 using ab155662 at a 1/200 dilution. The image in the lower panel was costained with Hoechst 33342.

References

ab155662 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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