Overview

  • Product name

    Anti-TKTL1/TKR antibody
  • Description

    Rabbit polyclonal to TKTL1/TKR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human TKTL1/TKR aa 1-311.
    Database link: P51854

  • Positive control

    • Recombinant Human TKTL1/TKR protein (ab152845) can be used as a positive control in WB. 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates; A431 cells; Human SAS xenograft tissue.
  • General notes

     This product was previously labelled as TKTL1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab155662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 65 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Tissue specificity

    Expressed in fetal and adult heart, brain, lung, liver, and kidney, and in adult placenta, skeletal muscle and pancreas. Up-regulated in various epithelial tumors.
  • Sequence similarities

    Belongs to the transketolase family.
  • Cellular localization

    Cytoplasm. Nucleus. Predominantly cytoplasmic and to a lesser extent also nuclear.
  • Information by UniProt
  • Database links

  • Alternative names

    • TK 2 antibody
    • TK2 antibody
    • TKR antibody
    • TKT 2 antibody
    • TKT2 antibody
    • TKTL1 antibody
    • TKTL1_HUMAN antibody
    • Transketolase 2 antibody
    • Transketolase like 1 antibody
    • Transketolase like protein 1 antibody
    • Transketolase related protein antibody
    • transketolase-like 1 antibody
    • Transketolase-like protein 1 antibody
    • Transketolase-related protein antibody
    • Transketolase2 antibody
    see all

Images

  • All lanes : Anti-TKTL1/TKR antibody (ab155662) at 1/5000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HepG2 whole cell lysate
    Lane 3 : MOLT4 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 65 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human SAS xenograft tissue, labeling TKTL1/TKR using ab155662 at a 1/100 dilution.

  • Immunofluorescence analysis of paraformaldehyde-fixed A431 cells, labeling TKTL1/TKR using ab155662 at a 1/200 dilution. The image in the lower panel was costained with Hoechst 33342.

References

This product has been referenced in:

  • Chiarini A  et al. Over Expressed TKTL1, CIP-2A, and B-MYB Proteins in Uterine Cervix Epithelium Scrapings as Potential Risk Predictive Biomarkers in HR-HPV-Infected LSIL/ASCUS Patients. Front Oncol 9:213 (2019). Read more (PubMed: 31001477) »
See 1 Publication for this product

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