Anti-TKTL1/TKR antibody (ab155662)
Key features and details
- Rabbit polyclonal to TKTL1/TKR
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TKTL1/TKR antibody -
Description
Rabbit polyclonal to TKTL1/TKR -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human TKTL1/TKR aa 1-311.
Database link: P51854 -
Positive control
- Recombinant Human TKTL1/TKR protein (ab152845) can be used as a positive control in WB. 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates; A431 cells; Human SAS xenograft tissue.
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General notes
This product was previously labelled as TKTL1
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155662 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 65 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
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| ICC/IF |
1/100 - 1/1000.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 65 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0). |
|
ICC/IF
1/100 - 1/1000. |
Target
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Tissue specificity
Expressed in fetal and adult heart, brain, lung, liver, and kidney, and in adult placenta, skeletal muscle and pancreas. Up-regulated in various epithelial tumors. -
Sequence similarities
Belongs to the transketolase family. -
Cellular localization
Cytoplasm. Nucleus. Predominantly cytoplasmic and to a lesser extent also nuclear. - Information by UniProt
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Database links
- Entrez Gene: 8277 Human
- Omim: 300044 Human
- SwissProt: P51854 Human
- Unigene: 102866 Human
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Alternative names
- TK 2 antibody
- TK2 antibody
- TKR antibody
see all
Images
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Western blot - Anti-TKTL1/TKR antibody (ab155662)All lanes : Anti-TKTL1/TKR antibody (ab155662) at 1/5000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : MOLT4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 65 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TKTL1/TKR antibody (ab155662)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human SAS xenograft tissue, labeling TKTL1/TKR using ab155662 at a 1/100 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-TKTL1/TKR antibody (ab155662)Immunofluorescence analysis of paraformaldehyde-fixed A431 cells, labeling TKTL1/TKR using ab155662 at a 1/200 dilution. The image in the lower panel was costained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab155662 has been referenced in 1 publication.
- Chiarini A et al. Over Expressed TKTL1, CIP-2A, and B-MYB Proteins in Uterine Cervix Epithelium Scrapings as Potential Risk Predictive Biomarkers in HR-HPV-Infected LSIL/ASCUS Patients. Front Oncol 9:213 (2019). PubMed: 31001477