Recombinant
Recombinant Anti-TKTL2 antibody [EPR8592] (ab131331)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-TKTL2 antibody [EPR8592]
See all TKTL2 primary antibodies -
Description
Rabbit monoclonal [EPR8592] to TKTL2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICCmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human TKTL2 aa 500-600. The exact sequence is proprietary.
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Positive control
- Jurkat, HepG2, BxPC 3 and MCF7 cell lysates; Human hepatocellular carcinoma tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.29 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8592 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab131331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC | 1/50 - 1/100. |
Application notes
Is unsuitable for Flow Cyt or IP.
Target
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Relevance
TKTL2 is an enzyme which catalyses the reaction Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate, which may link the pentose phosphate and glycolysis pathways. -
Database links
- Entrez Gene: 84076 Human
- Entrez Gene: 74419 Mouse
- Entrez Gene: 290685 Rat
- SwissProt: Q9H0I9 Human
- SwissProt: Q9D4D4 Mouse
- Unigene: 303923 Human
- Unigene: 483407 Mouse
- Unigene: 124739 Rat
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Alternative names
- DKFZp434L1717 antibody
- FLJ32975 antibody
- TKTL2 antibody
see all
Images
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![Western blot - Anti-TKTL2 antibody [EPR8592] (ab131331)](https://www.abcam.com/ps/products/131/ab131331/Images/ab131331-145719-anti-tktl2-antibody-epr8592-western-blot.jpg)
Western blot - Anti-TKTL2 antibody [EPR8592] (ab131331)All lanes : Anti-TKTL2 antibody [EPR8592] (ab131331) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : BxPC 3 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 68 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TKTL2 antibody [EPR8592] (ab131331)](https://www.abcam.com/ps/products/131/ab131331/Images/ab131331-145718-anti-tktl2-antibody-epr8592-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TKTL2 antibody [EPR8592] (ab131331)Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labelling TKTL2 with ab131331 at 1/100 dilution. -
![Other - Anti-TKTL2 antibody [EPR8592] (ab131331)](https://www.abcam.com/ps/products/131/ab131331/Images/ab131331-145717-anti-tktl2-antibody-epr8592.jpg)
Other - Anti-TKTL2 antibody [EPR8592] (ab131331)
Protocols
Datasheets and documents
References
ab131331 has not yet been referenced specifically in any publications.
