Recombinant Anti-TKTL2 antibody [EPR8592] (ab131331)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8592] to TKTL2
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-TKTL2 antibody [EPR8592]
See all TKTL2 primary antibodies -
Description
Rabbit monoclonal [EPR8592] to TKTL2 -
Host species
Rabbit -
Tested Applications & Species
Application Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human TKTL2 aa 500-600. The exact sequence is proprietary.
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Positive control
- Jurkat, HepG2, BxPC 3 and MCF7 cell lysates; Human hepatocellular carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.29 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8592 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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IHC-P |
Human
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WB |
Human
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All applications |
Mouse
Rat
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Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Relevance
TKTL2 is an enzyme which catalyses the reaction Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate, which may link the pentose phosphate and glycolysis pathways. -
Database links
- Entrez Gene: 84076 Human
- Entrez Gene: 74419 Mouse
- Entrez Gene: 290685 Rat
- SwissProt: Q9H0I9 Human
- SwissProt: Q9D4D4 Mouse
- Unigene: 303923 Human
- Unigene: 483407 Mouse
- Unigene: 124739 Rat
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Alternative names
- DKFZp434L1717 antibody
- FLJ32975 antibody
- TKTL2 antibody
see all
Images
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All lanes : Anti-TKTL2 antibody [EPR8592] (ab131331) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : BxPC 3 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 68 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TKTL2 antibody [EPR8592] (ab131331)
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labelling TKTL2 with ab131331 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References (0)
ab131331 has not yet been referenced specifically in any publications.