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RecombinantRabMAb

Recombinant Anti-TKTL2 antibody [EPR8592] (ab131331)

  • Datasheet
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Western blot - Anti-TKTL2 antibody [EPR8592] (ab131331)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TKTL2 antibody [EPR8592] (ab131331)
  • OI-RD Scanning - Anti-TKTL2 antibody [EPR8592] (ab131331)
  • Anti-TKTL2 antibody [EPR8592] (ab131331)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8592] to TKTL2
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

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Overview

  • Product name

    Anti-TKTL2 antibody [EPR8592]
    See all TKTL2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8592] to TKTL2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human TKTL2 aa 500-600. The exact sequence is proprietary.

  • Positive control

    • Jurkat, HepG2, BxPC 3 and MCF7 cell lysates; Human hepatocellular carcinoma tissue.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 2.29 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8592
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Alternative Versions

    • Anti-TKTL2 antibody [EPR8592] - BSA and Azide free (ab248410)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab131331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Relevance

      TKTL2 is an enzyme which catalyses the reaction Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate, which may link the pentose phosphate and glycolysis pathways.
    • Database links

      • Entrez Gene: 84076 Human
      • Entrez Gene: 74419 Mouse
      • Entrez Gene: 290685 Rat
      • SwissProt: Q9H0I9 Human
      • SwissProt: Q9D4D4 Mouse
      • Unigene: 303923 Human
      • Unigene: 483407 Mouse
      • Unigene: 124739 Rat
      • Alternative names

        • DKFZp434L1717 antibody
        • FLJ32975 antibody
        • TKTL2 antibody
        • Transketolase like 2 antibody
        • Transketolase-like protein 2 antibody
        see all

      Images

      • Western blot - Anti-TKTL2 antibody [EPR8592] (ab131331)
        Western blot - Anti-TKTL2 antibody [EPR8592] (ab131331)
        All lanes : Anti-TKTL2 antibody [EPR8592] (ab131331) at 1/1000 dilution

        Lane 1 : Jurkat cell lysate
        Lane 2 : HepG2 cell lysate
        Lane 3 : BxPC 3 cell lysate
        Lane 4 : MCF7 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 68 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TKTL2 antibody [EPR8592] (ab131331)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TKTL2 antibody [EPR8592] (ab131331)

        Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labelling TKTL2 with ab131331 at 1/100 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • OI-RD Scanning - Anti-TKTL2 antibody [EPR8592] (ab131331)
        OI-RD Scanning - Anti-TKTL2 antibody [EPR8592] (ab131331)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-TKTL2 antibody [EPR8592] (ab131331)
        Anti-TKTL2 antibody [EPR8592] (ab131331)

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab131331? Please let us know so that we can cite the reference in this datasheet.

      ab131331 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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