Overview

  • Product name
  • Description
    Rabbit polyclonal to TLE 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to a region within internal sequence amino acids 231-479 of Human TLE 1 (UniProt ID: Q04724).

  • Positive control
    • 293T and A431 whole cell lysate; A431 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab155046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 83 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.
  • Tissue specificity
    In all tissues examined, mostly in brain, liver and muscle.
  • Sequence similarities
    Belongs to the WD repeat Groucho/TLE family.
    Contains 6 WD repeats.
  • Domain
    WD repeat Groucho/TLE family members are characterized by 5 regions, a glutamine-rich Q domain, a glycine/proline-rich GP domain, a central CcN domain, containing a nuclear localization signal, and a serine/proline-rich SP domain. The most highly conserved are the N-terminal Q domain and the C-terminal WD-repeat domain.
  • Post-translational
    modifications
    Phosphorylated, probably by CDK1. The degree of phosphorylation varies throughout the cell cycle, and is highest at the G2/M transition. Becomes hyperphosphorylated in response to cell differentiation and interaction with HES1 or RUNX1.
    Ubiquitinated by XIAP/BIRC4.
  • Cellular localization
    Nucleus. Nuclear and chromatin-associated, depending on isoforms and phosphorylation status. Hyperphosphorylation decreases the affinity for nuclear components.
  • Information by UniProt
  • Database links
  • Alternative names
    • E(Sp1) homolog antibody
    • Enhancer of split groucho 1 antibody
    • Enhancer of split groucho like protein 1 antibody
    • Enhancer of split groucho-like protein 1 antibody
    • ESG antibody
    • ESG1 antibody
    • GRG1 antibody
    • TLE1 antibody
    • TLE1_HUMAN antibody
    • Transducin like enhancer of split 1 (E(sp1) homolog Drosophila) antibody
    • Transducin like enhancer of split 1 antibody
    • Transducin like enhancer of split 1 homolog of Drosophila E(sp1) antibody
    • Transducin like enhancer protein 1 antibody
    • Transducin-like enhancer protein 1 antibody
    see all

Images

  • Anti-TLE 1 antibody (ab155046) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 83 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde fixed A431 cels labeling TLE 1 with ab155046 at 1/500 dilution. Lower image costained with Hoerchst 33342.

References

This product has been referenced in:
  • Yao X  et al. TLE1 inhibits anoikis and promotes tumorigenicity in human lung cancer cells through ZEB1-mediated E-cadherin repression. Oncotarget 8:72235-72249 (2017). Read more (PubMed: 29069783) »
  • Liu J  et al. Trichostatin A suppresses lung adenocarcinoma development in Grg1 overexpressing transgenic mice. Biochem Biophys Res Commun 463:1230-6 (2015). WB . Read more (PubMed: 26086099) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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