Overview

  • Product name

    Anti-TLE 1 antibody [OTI1F5]
    See all TLE 1 primary antibodies
  • Description

    Mouse monoclonal [OTI1F5] to TLE 1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 180-460 of Human TLE 1 produced in E.coli (NP_005068).

  • Positive control

    • Human breast adenocarcinoma, colon, kidney, kidney carcinoma, lung carcinoma, ovary, ovary carcinoma, pancreas, thyroid carcinoma, endometrium adenocarcinoma, prostate, prostate carcinoma, bladder carcinoma, lymphoma and tonsil tissues; HEK293T cells transfected with pCMV6-ENTRY TLE 1 cDNA; COS7 cells transiently transfected by pCMV6-ENTRY TLE1; HEK293T cells transfected with pCMV6-ENTRY TLE 1 overexpress plasmid.
  • General notes

    Dilute in PBS (pH7.3) before use.

    The clone number has been updated from 1F5 to OTI1F5, both clone numbers name the same clone.

    This product was changed from ascites to tissue culture supernatant on 6th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7,30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    ab131648 is purified from TCS by affinity chromatography.
  • Clonality

    Monoclonal
  • Clone number

    OTI1F5
  • Isotype

    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab131648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 83 kDa.
IHC-P 1/150.
Flow Cyt 1/100.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100.

Target

  • Function

    Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.
  • Tissue specificity

    In all tissues examined, mostly in brain, liver and muscle.
  • Sequence similarities

    Belongs to the WD repeat Groucho/TLE family.
    Contains 6 WD repeats.
  • Domain

    WD repeat Groucho/TLE family members are characterized by 5 regions, a glutamine-rich Q domain, a glycine/proline-rich GP domain, a central CcN domain, containing a nuclear localization signal, and a serine/proline-rich SP domain. The most highly conserved are the N-terminal Q domain and the C-terminal WD-repeat domain.
  • Post-translational
    modifications

    Phosphorylated, probably by CDK1. The degree of phosphorylation varies throughout the cell cycle, and is highest at the G2/M transition. Becomes hyperphosphorylated in response to cell differentiation and interaction with HES1 or RUNX1.
    Ubiquitinated by XIAP/BIRC4.
  • Cellular localization

    Nucleus. Nuclear and chromatin-associated, depending on isoforms and phosphorylation status. Hyperphosphorylation decreases the affinity for nuclear components.
  • Information by UniProt
  • Database links

  • Alternative names

    • E(Sp1) homolog antibody
    • Enhancer of split groucho 1 antibody
    • Enhancer of split groucho like protein 1 antibody
    • Enhancer of split groucho-like protein 1 antibody
    • ESG antibody
    • ESG1 antibody
    • GRG1 antibody
    • TLE1 antibody
    • TLE1_HUMAN antibody
    • Transducin like enhancer of split 1 (E(sp1) homolog Drosophila) antibody
    • Transducin like enhancer of split 1 antibody
    • Transducin like enhancer of split 1 homolog of Drosophila E(sp1) antibody
    • Transducin like enhancer protein 1 antibody
    • Transducin-like enhancer protein 1 antibody
    see all

Images

  • All lanes : Anti-TLE 1 antibody [OTI1F5] (ab131648) at 1/2000 dilution

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY TLE1 cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 83 kDa



    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC204037)
  • Flow cytometric analysis of HEK293T cells transfected with either pCMV6-ENTRY TLE1 (red) or empty vector control plasmid (blue) labelling TLE1 with ab131648 at 1/100 dilution.
  • Immunofluorescent analysis of COS7 cells transiently transfected by pCMV6-ENTRY TLE1 labelling TLE1 with ab131648 at 1/100 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling TLE1 with ab131648 at 1/150 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human breast tissue labelling TLE1 with ab131648 at 1/150 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human breast adenocarcinoma tissue labelling TLE1 with ab131648 at 1/150 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling TLE1 with ab131648 at 1/150 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labelling TLE1 with ab131648 at 1/150 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling TLE1 with ab131648 at 1/150 dilution.
  • Immunohistochemical analysis of paraffin-embedded Carcinoma of Human kidney tissue labelling TLE1 with ab131648 at 1/150 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling TLE1 with ab131648 at 1/150 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human ovary tissue labelling TLE1 with ab131648 at 1/150 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human ovary adenocarcinoma tissue labelling TLE1 with ab131648 at 1/150 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labelling TLE1 with ab131648 at 1/150 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labelling TLE1 with ab131648 at 1/150 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labelling TLE1 with ab131648 at 1/150 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human prostate tissue labelling TLE1 with ab131648 at 1/150 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human prostate carcinoma tissue labelling TLE1 with ab131648 at 1/150 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human bladder carcinoma tissue labelling TLE1 with ab131648 at 1/150 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human lymphoma tissue labelling TLE1 with ab131648 at 1/150 dilution.

References

ab131648 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
IHC - Wholemount
Sample
Chicken Embryo (Whole embryo - stage HH9-10)
Specification
Whole embryo - stage HH9-10

Abcam user community

Verified customer

Submitted Aug 22 2018

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