Product nameTLE2 overexpression 293T lysate (whole cell)
ab94118 is a 293T cell transfected lysate in which Human TLE2 has been transiently over-expressed using a pCMV-TLE2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 10% Glycerol (glycerin, glycerine), 0.788% Tris HCl, 2% Sodium lauryl sulfate, 2.3% Beta mercaptoethanol, 0.01% Bromophenol blue
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BackgroundFunction: Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Tissue specificity: In all tissues examined, mostly in heart, brain, and muscle. Similarity: Belongs to the WD repeat Groucho/TLE family. Contains 6 WD repeats.
Our Abpromise guarantee covers the use of ab94118 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94118 at 15μg/lane on an SDS-PAGE gel
All lanes : Anti-TLE2 antibody (ab72634) at 1/500 dilution
Lane 1 :
TLE2 overexpression 293T lysate (whole cell) (ab94118)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg/ml per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94118 has not yet been referenced specifically in any publications.