Key features and details
- Rabbit polyclonal to TLE3/ESG
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TLE3/ESG antibody
See all TLE3/ESG primary antibodies
DescriptionRabbit polyclonal to TLE3/ESG
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- Human vulva/anal skin tissue.
Previously labelled as TLE3.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab185508 in the following tested applications.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 83 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionTranscriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES.
Tissue specificityPlacenta and lung.
Sequence similaritiesBelongs to the WD repeat Groucho/TLE family.
Contains 7 WD repeats.
modificationsUbiquitinated by XIAP/BIRC4. This ubiquitination does not affect its stability, nuclear localization, or capacity to tetramerize but inhibits its interaction with TCF7L2/TCF4.
- Information by UniProt
- E(sp1) homolog Drosophila antibody
- Enhancer of split groucho 3 antibody
- Enhancer of split groucho-like protein 3 antibody
Anti-TLE3/ESG antibody (ab185508) + HeLa nuclear extract
Predicted band size: 83 kDa
Immunohistochemical analysis of paraffin-embedded Human vulva/anal skin tissue, labeling TLE3/ESG using ab185508 at a 1/500 dilution.
Immunofluorescent staining of human cell line HeLa staining TLE3/ESG with ab185508. Antibody staining is shown in green
ab185508 has not yet been referenced specifically in any publications.