Overview

  • Product name

    Anti-TLE3/ESG antibody [CL3573]
    See all TLE3/ESG primary antibodies
  • Description

    Mouse monoclonal [CL3573] to TLE3/ESG
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human TLE3/ESG aa 173-215.
    Sequence:

    GSQAHLTVKDEKNHHELDHRERESSANNSVSPSESLRASEKHR


    Database link: Q04726

  • Positive control

    • HEK293 cell lysate and HeLa nuclear lysate; human cervix, tonsil, oral mucosa, small intestine, kidney and skeletal muscle tissues.
  • General notes

    Previously labelled as TLE3. 

Properties

Applications

Our Abpromise guarantee covers the use of ab213596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 83 kDa.
IHC-P 1/10000 - 1/20000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES.
  • Tissue specificity

    Placenta and lung.
  • Sequence similarities

    Belongs to the WD repeat Groucho/TLE family.
    Contains 7 WD repeats.
  • Post-translational
    modifications

    Ubiquitinated by XIAP/BIRC4. This ubiquitination does not affect its stability, nuclear localization, or capacity to tetramerize but inhibits its interaction with TCF7L2/TCF4.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • E(sp1) homolog Drosophila antibody
    • Enhancer of split groucho 3 antibody
    • Enhancer of split groucho-like protein 3 antibody
    • ESG 3 antibody
    • ESG antibody
    • ESG3 antibody
    • FLJ39460 antibody
    • GRG 3 antibody
    • GRG3 antibody
    • HsT18976 antibody
    • KIAA1547 antibody
    • TLE 3 antibody
    • Tle3 antibody
    • TLE3_HUMAN antibody
    • Transducin like enhancer of split 3 antibody
    • Transducin like enhancer of split 3 homolog of Drosophila E(sp1) antibody
    • Transducin like enhancer protein 3 antibody
    • transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila) antibody
    • Transducin-like enhancer protein 3 antibody
    see all

Images

  • Immunohistochemical analysis of Paraffin-embedded Human cervix tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.

  • Immunohistochemical analysis of Paraffin-embedded Human tonsil tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.

  • Immunohistochemical analysis of Paraffin-embedded Human oral mucosa tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.

  • Immunohistochemical analysis of Paraffin-embedded Human small intestine tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.

  • Immunohistochemical analysis of Paraffin-embedded Human kidney tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.

  • Immunohistochemical analysis of Paraffin-embedded Human skeletal muscle tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.

  • Anti-TLE3/ESG antibody [CL3573] (ab213596) at 1/500 dilution + HEK293 cell lysate

    Predicted band size: 83 kDa

  • Anti-TLE3/ESG antibody [CL3573] (ab213596) at 1/500 dilution + HeLa nuclear lysate

    Predicted band size: 83 kDa

References

This product has been referenced in:

  • Pearson S  et al. Loss of TLE3 promotes the mitochondrial program in beige adipocytes and improves glucose metabolism. Genes Dev 33:747-762 (2019). Read more (PubMed: 31123067) »
  • Kokabu S  et al. The transcriptional co-repressor TLE3 regulates myogenic differentiation by repressing the activity of the MyoD transcription factor. J Biol Chem 292:12885-12894 (2017). Read more (PubMed: 28607151) »
See all 2 Publications for this product

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