Anti-TLE3/ESG antibody [CL3573] (ab213596)
Key features and details
- Mouse monoclonal [CL3573] to TLE3/ESG
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-TLE3/ESG antibody [CL3573]
See all TLE3/ESG primary antibodies -
Description
Mouse monoclonal [CL3573] to TLE3/ESG -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human TLE3/ESG aa 150-250.
Database link: Q04726 -
Positive control
- WB: HEK293 cell lysate and HeLa nuclear lysate. IHC-P: Human cervix, tonsil, oral mucosa, small intestine, kidney and skeletal muscle tissues. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL3573 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab213596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 83 kDa.
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IHC-P |
1/10000 - 1/20000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 2 - 10 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 83 kDa. |
IHC-P
1/10000 - 1/20000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 2 - 10 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
Target
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Function
Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. -
Tissue specificity
Placenta and lung. -
Sequence similarities
Belongs to the WD repeat Groucho/TLE family.
Contains 7 WD repeats. -
Post-translational
modificationsUbiquitinated by XIAP/BIRC4. This ubiquitination does not affect its stability, nuclear localization, or capacity to tetramerize but inhibits its interaction with TCF7L2/TCF4. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7090 Human
- Entrez Gene: 21887 Mouse
- Entrez Gene: 84424 Rat
- Omim: 600190 Human
- SwissProt: Q04726 Human
- SwissProt: Q08122 Mouse
- SwissProt: Q9JIT3 Rat
- Unigene: 287362 Human
see all -
Alternative names
- E(sp1) homolog Drosophila antibody
- Enhancer of split groucho 3 antibody
- Enhancer of split groucho-like protein 3 antibody
see all
Images
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PFA-fixed, Triton X-100 permeabilized HeLa cells stained for TLE3 (Green) using ab213596 at 4 μg/ml in ICC/IF.
Microtublues are stained red.
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Anti-TLE3/ESG antibody [CL3573] (ab213596) at 1/500 dilution + HEK293 cell lysate
Predicted band size: 83 kDa -
Immunohistochemical analysis of Paraffin-embedded Human cervix tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.
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Immunohistochemical analysis of Paraffin-embedded Human tonsil tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.
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Immunohistochemical analysis of Paraffin-embedded Human oral mucosa tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.
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Immunohistochemical analysis of Paraffin-embedded Human small intestine tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.
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Immunohistochemical analysis of Paraffin-embedded Human kidney tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.
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Immunohistochemical analysis of Paraffin-embedded Human skeletal muscle tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.
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Anti-TLE3/ESG antibody [CL3573] (ab213596) at 1/500 dilution + HeLa nuclear lysate
Predicted band size: 83 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab213596 has been referenced in 2 publications.
- Pearson S et al. Loss of TLE3 promotes the mitochondrial program in beige adipocytes and improves glucose metabolism. Genes Dev 33:747-762 (2019). PubMed: 31123067
- Kokabu S et al. The transcriptional co-repressor TLE3 regulates myogenic differentiation by repressing the activity of the MyoD transcription factor. J Biol Chem 292:12885-12894 (2017). PubMed: 28607151