Product nameAnti-TLE4 antibody
See all TLE4 primary antibodies
DescriptionRabbit polyclonal to TLE4
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Rabbit, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide corresponding to Mouse TLE4 aa 200-300 conjugated to keyhole limpet haemocyanin.
Database link: Q62441
- This antibody gave a positive signal in the following tissue lysates: P7 Mouse Brain; P0 Mouse Brain; E16 Embryonic Mouse Brain; E16 Embryonic Rat Brain; E14 Embryonic Mouse Brain; E14 Embryonic Rat Brain; IHC-P: FFPE mouse brain E17 FFPE tissue sections.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab140485 in the following tested applications.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa).|
RelevanceTLE4 (Transducin-like enhancer protein 4) is a member of the Groucho family of transcriptional corepressors and inhibits the transcriptional activation mediated by Pax5 and by beta-catenin and TCF family members in Wnt signaling.
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IHC image of TLE4 staining in mouse brain E17 formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab140485, 1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-TLE4 antibody (ab140485) at 1 µg/ml
Lane 1 : P7 Mouse Brain Tissue Lysate Lab Lysate
Lane 2 : P0 Mouse Brain Mouse Tissue Lysate
Lane 3 : E16 Ms Embryo Brain Tissue Lysate
Lane 4 : E16 Rat Embryo Brain Tissue Lysate
Lane 5 : E14 Mouse Embryo Brain Tissue Lysate
Lane 6 : E14 Rat Embryo Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa (possible non-specific binding), 34 kDa (possible non-specific binding), 56 kDa (possible non-specific binding)
Exposure time: 30 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab140485 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab140485 has not yet been referenced specifically in any publications.