Anti-TLK1 antibody (ab155823)
Key features and details
- Rabbit polyclonal to TLK1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TLK1 antibody
See all TLK1 primary antibodies -
Description
Rabbit polyclonal to TLK1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Recombinant fragment corresponding to a region within internal sequence amino acids 237-625 of Human TLK1 (UniProt ID: Q9UKI8).
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Positive control
- 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji whole cell lysate. HeLa cells.
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General notes
Store as concentrated solution.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab155823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 87 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by faciliting the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'. -
Tissue specificity
Widely expressed. Present in fetal placenta, liver, kidney and pancreas but not heart or skeletal muscle. Also found in adult cell lines. Isoform 3 is ubiquitously expressed in all tissues examined. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9874 Human
- Entrez Gene: 228012 Mouse
- Omim: 608438 Human
- SwissProt: Q9UKI8 Human
- SwissProt: Q8C0V0 Mouse
- Unigene: 726093 Human
- Unigene: 744917 Human
- Unigene: 136511 Mouse
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Alternative names
- PKU beta antibody
- PKU-beta antibody
- serine threonine protein kinase antibody
see all
Images
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Western blot - Anti-TLK1 antibody (ab155823)Anti-TLK1 antibody (ab155823) at 1/500 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 87 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-TLK1 antibody (ab155823)Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, labeling TLK1 using ab155823 at 1/200 dilution. Lower image merged with DNA probe.
Protocols
References (0)
ab155823 has not yet been referenced specifically in any publications.
