Anti-TLK1 antibody (ab155823)

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Overview

  • Product name

    Anti-TLK1 antibody
    See all TLK1 primary antibodies

  • Description

    Rabbit polyclonal to TLK1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Recombinant fragment corresponding to a region within internal sequence amino acids 237-625 of Human TLK1 (UniProt ID: Q9UKI8).

  • Positive control

    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji whole cell lysate. HeLa cells.

  • General notes

    Store as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab155823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 87 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by faciliting the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'.

  • Tissue specificity

    Widely expressed. Present in fetal placenta, liver, kidney and pancreas but not heart or skeletal muscle. Also found in adult cell lines. Isoform 3 is ubiquitously expressed in all tissues examined.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • PKU beta antibody
      • PKU-beta antibody
      • serine threonine protein kinase antibody
      • Serine/threonine protein kinase tousled like 1 antibody
      • Serine/threonine-protein kinase tousled-like 1 antibody
      • SNARE protein kinase SNAK antibody
      • Tlk1 antibody
      • TLK1_HUMAN antibody
      • Tousled like kinase 1 antibody
      • Tousled-like kinase 1 antibody
      see all

    Images

    • Western blot - Anti-TLK1 antibody (ab155823)
      Western blot - Anti-TLK1 antibody (ab155823)
      Anti-TLK1 antibody (ab155823) at 1/500 dilution + 293T whole cell lysate at 30 µg

      Predicted band size: 87 kDa



      7.5% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-TLK1 antibody (ab155823)
      Immunocytochemistry/ Immunofluorescence - Anti-TLK1 antibody (ab155823)
      Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, labeling TLK1 using ab155823 at 1/200 dilution. Lower image merged with DNA probe.

    References

    ab155823 has not yet been referenced specifically in any publications.

    Publishing research using ab155823? Please let us know so that we can cite the reference in this datasheet.

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