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    tlk1-antibody-ab155823.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Phosphorylation
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Anti-TLK1 antibody (ab155823)

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  • SDS
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Western blot - Anti-TLK1 antibody (ab155823)
  • Immunocytochemistry/ Immunofluorescence - Anti-TLK1 antibody (ab155823)

Key features and details

  • Rabbit polyclonal to TLK1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Protein
Product image
Recombinant human TLK1 protein (ab125563)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-TLK1 antibody
    See all TLK1 primary antibodies
  • Description

    Rabbit polyclonal to TLK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment corresponding to a region within internal sequence amino acids 237-625 of Human TLK1 (UniProt ID: Q9UKI8).

  • Positive control

    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji whole cell lysate. HeLa cells.
  • General notes

    Store as concentrated solution.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Phosphorylation
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • A431 whole cell lysate (ab30132)
    • A431 whole cell lysate (ab7909)
    • MOLT4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant human TLK1 protein (ab125563)
  • Related Products

    • Recombinant human TLK1 protein (ab125563)

Applications

Our Abpromise guarantee covers the use of ab155823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 87 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by faciliting the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'.
  • Tissue specificity

    Widely expressed. Present in fetal placenta, liver, kidney and pancreas but not heart or skeletal muscle. Also found in adult cell lines. Isoform 3 is ubiquitously expressed in all tissues examined.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q9UKI8 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9874 Human
    • Entrez Gene: 228012 Mouse
    • Omim: 608438 Human
    • SwissProt: Q9UKI8 Human
    • SwissProt: Q8C0V0 Mouse
    • Unigene: 726093 Human
    • Unigene: 744917 Human
    • Unigene: 136511 Mouse
    • Alternative names

      • PKU beta antibody
      • PKU-beta antibody
      • serine threonine protein kinase antibody
      • Serine/threonine protein kinase tousled like 1 antibody
      • Serine/threonine-protein kinase tousled-like 1 antibody
      • SNARE protein kinase SNAK antibody
      • Tlk1 antibody
      • TLK1_HUMAN antibody
      • Tousled like kinase 1 antibody
      • Tousled-like kinase 1 antibody
      see all

    Images

    • Western blot - Anti-TLK1 antibody (ab155823)
      Western blot - Anti-TLK1 antibody (ab155823)
      Anti-TLK1 antibody (ab155823) at 1/500 dilution + 293T whole cell lysate at 30 µg

      Predicted band size: 87 kDa



      7.5% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-TLK1 antibody (ab155823)
      Immunocytochemistry/ Immunofluorescence - Anti-TLK1 antibody (ab155823)
      Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, labeling TLK1 using ab155823 at 1/200 dilution. Lower image merged with DNA probe.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab155823? Please let us know so that we can cite the reference in this datasheet.

    ab155823 has not yet been referenced specifically in any publications.

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